Simultaneous measurement of blood coagulation and erythrocyte sedimentation by means of a rheological technique
Tóm tắt
With a damped-oscillation rheometer, changes in the rheological properties, i.e., logarithmic damping factor (LDF) and period, as obtained from a damped-oscillation curve, were monitored during the coagulation of blood. In our earlier studies, the time of onset of coagulation (Ti) of the blood sample was only determined from the change in LDF. When coagulation of the blood and sedimentation of erythrocytes occurred together, the Ti value could not be determined from the change in LDF. In this paper, a method for determining the Ti value from the change in the period of the damped-oscillation curve was investigated. It was found that the period increased and leveled off as blood coagulation progressed, and the Ti value was determined from the middle point between the minimum and maximum values of the period. In addition, it was suggested that the level of erythrocyte sedimentation could be estimated from the initial decrease in LDF. In blood obtained from diabetic patients, a good correlation between the initial decrease in the LDF and the concentration of fibrinogen was observed. Our study demonstrates that when erythrocyte sedimentation and blood coagulation occur simultaneously, this rheological technique makes it possible to measure the Ti value and erythrocyte sedimentation.
Tài liệu tham khảo
Kirkpatrick JP, McIntire LV, Moake JL, Peterson DL. Dynamic rheological studies of coagulation and fibrinolysis. J Rheol. 1979;23:769–87.
Hertert H. Thrombelastography: physical and physiological aspects. In: Copley AL, Stainsby G, Scott Blair GW, editors. Flow properties of blood and other biological systems. Oxford: Pergamon Press; 1960. p. 186–98.
Kaibara M. Rheological studies on blood coagulation and network formation of fibrin. Polym Gels Networks. 1994;2:1–28.
Kawamoto Y, Kaibara M. Viscoelasticity of fibrinogen solution and of blood during coagulation studied by a new damped oscillation rheometer. Biorheology. 1988;25:289–96.
Kawakami S, Kaibara M, Kawamoto Y, Yamanaka K. Rheological approach to the analysis of blood coagulation in endothelial cell-coated tubes: activation of the intrinsic reaction on the erythrocyte surface. Biorheology. 1995;32:521–36.
Kawakami S, Isogai Y, Yamamoto J, Maeda T, Ikemoto S, Kaibara M. Rheological study on erythrocyte aggregation with special reference to ESR—application to quick estimation of ESR value. Clin Hemorheol. 1994;14:509–18.
Kaibara M, Iwata H, Ujiie H, Himeno R, Kaibara M. Rheological analyses of coagulation of blood from different individuals with special reference to procoagulant activity of erythrocytes. Blood Coagul Fibrinolysis. 2005;16:355–63.
Rheinhart WH, Lutolf O, Nydegger UR, Mahler F, Straub PW. Plasmapheresis for hyperviscosity syndrome in macroglobulinemia Waldenstrom and multiple myeloma: influence on blood rheology and the microcirculation. J Lab Clin Med. 1992;119:69–76.
Dintenfass L. Blood microrheology—viscosity factors in blood flow, ischaemia and thrombosis. London: Butterworths; 1971.
Kanai I, Kanai M. Rinsyokensahouteiyou (summary of clinical examination methods). Tokyo: Kinbarashuppan; 1978. (in Japanese).
Kaibara M, Date M. A new rheological method to measure fluidity change of blood during coagulation. Application to in vitro evaluation of anticoagulability of artificial materials. Biorheology. 1985;22:197–208.
Murata T, Date M, Kaibara M. Analysis of a newly developed damped-oscillation rheometer: Newtonian liquid. Jpn J Appl Phys. 1987;26:1234–40.
Kaibara M. Rheological study on coagulation of blood with special reference to the triggering mechanism of venous thrombus formation. J Biorheol. 2009;23:2–10.
Hager K, Setzer J, Vogl T, Voit J, Platt D. Blood coagulation factors in the elderly. Arch Gerontol Geriatr. 1989;9:277–82.
Ujiie H, Kawasaki M, Suzuki Y, Kaibara M. Influence of age and hematocrit on the coagulation of blood. J Biorheol. 2009;23:111–4.
Shiga T, Maeda N, Kon K. Erythrocyte rheology. Crit Rev Oncol Hematol. 1990;10:9–48.
Isogai Y, Iida A, Chikatsu I, Mochizuki K, Abe M. Dynamic viscoelasticity of blood during clotting in health and disease. Biorheology. 1973;10:411–24.
Shiga T, Imaizumi K, Harada N, Sekiya M. Kinetics of rouleaux formation using TV image analyzer. I. Human erythrocytes. Am J Physiol. 1983;245:H252–8.
Westergren A. The technique of the red blood cell sedimentation reaction. Am Rev Tuberc. 1926;14:94–101.
Isogai Y, Ikemoto S, Tanaka H. Elevation of viscoelasticity of blood in non-coagulative and coagulative state in diabetes, as indices of increasing the incidence of aggravation of microangiopathy. Clin Hemorheol. 1996;16:713–35.
Isogai Y, Mochizuki K, Yokose T, Okabe H, Ashikaga M, Akiyama M, Abe M. Intravascular erythrocyte aggregation and hemorheological abnormalities in diabetes. Bibl Anat. 1981;20:161–5.
Iwata H, Kaibara M. Activation of factor IX by erythrocyte membranes causes intrinsic coagulation. Blood Coagul Fibrinolysis. 2002;13:489–96.