Simultaneous determination of four antiepileptic drugs in serum by high‐performance liquid chromatography

Biomedical Chromatography - Tập 16 Số 1 - Trang 19-24 - 2002
H. Levert1, Pascal Odou2, H. Robert2,1
1EPSM Lille-métropole Hospital, Lille, France
2Department of Biopharmacy and Clinical Pharmacy, Faculty of Pharmaceutical and Biological Sciences, Lille II University, Lille, France

Tóm tắt

AbstractA high‐performance liquid chromatographic method has been developed for the simultaneous determination of oxcarbazepine, 10‐hydroxycarbamazepine, epoxycarbamazepine, carbamazepine, phenobarbital and phenytoin. After protein precipitation by acetonitrile, the supernatant was analysed on a C18 reversed‐phase HPLC column. Antiepileptic drugs and oxazepam (internal standard) were detected by ultraviolet absorbance at 240 nm. Linearity was established for the whole concentration range ­for each compound. Quantitation limits of oxcarbazepine, 10‐hydroxycarbamazepine, epoxycarbamazepine, carbamazepine, phenobarbital and phenytoin were 0.58, 3.5, 2.35, 0.66, 1.02 and 3.13 µg/mL, respectively, and mean recoveries added to serum were 105.15, 84.76, 94,45, 96.52, 98.62 and 95.08%, respectively. This method has been used for the simultaneous determination of steady‐state serum concentration of antiepileptic drugs in patients treated by one or more anticonvulsive treatment. Copyright © 2001 John Wiley & Sons, Ltd.

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Biomedical Chromatography

Tập 16 Số 1

19-24

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