Simplifying sample preparation using fabric phase sorptive extraction technique for the determination of benzodiazepines in blood serum by high‐performance liquid chromatography

Biomedical Chromatography - Tập 30 Số 6 - Trang 829-836 - 2016
Victoria Samanidou1, Ioanna Kaltzi1, Abuzar Kabir2, Kenneth G. Furton2
1Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, Greece
2International Forensic Research Institute, Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA

Tóm tắt

AbstractFabric phase sorptive extraction (FPSE), a recently introduced novel sample preparation technology, has been evaluated for the extraction of benzodiazepines from human blood serum. FPSE utilizes a flexible fabric surface as the substrate platform for creating sol‐gel hybrid organic‐inorganic sorbent coatings. FPSE media can be introduced directly into the sample containing the target analyte(s), requiring no need for prior sample pretreatment or clean‐up. Benzodiazepines were selected as model analytes because they represent one of the most widely used therapeutic drugs in psychiatry and are also amongst the most frequently encountered drugs in forensic toxicology. The chromatographic separation of target analytes was performed on a LiChroCART‐LiChrospher®100 RP‐18e (5 µm, 250 × 4 mm) analytical column, operated at room temperature. Ternary gradient elution was applied with a mobile phase that consisted of acetonitrile, methanol and ammonium acetate (0.05 M), which was delivered at a flow rate of 1.0 mL/min. Diode array detection was performed with monitoring at 240 nm. FPSE was performed using cellulose fabric extraction media coated with sol‐gel poly(ethylene glycol) (sol‐gel PEG). Absolute recovery values in the equilibrium state for the examined benzodiazepines were found to be 27% for bromazepam, 63% for lorazepam, 42 % for diazepam and 39% for alprazolam. Copyright © 2015 John Wiley & Sons, Ltd.

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Biomedical Chromatography

Tập 30 Số 6

829-836

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