Simple strategy for the in vitro conservation of Alnus glutinosa (L.) Gaertn. germplasm
Tóm tắt
This study provides a simple protocol for storage of alder clones. The technique described is useful for in vitro germplasm collections, decreasing the risk of genetic changes and associated costs.
The aim of this study was to develop a simple method for the medium-term storage of Alnus glutinosa (L.) Gaertn. explants obtained from trees aged 20–30 years. Several parameters were evaluated, including type of explant (shoot apex or nodal segments), pre-storage treatment (0 or 10 days after the last subculture) and duration of cold storage (3–24 months) at 2–4 °C. Explants were maintained at this temperature under dim lighting on Woody Plant Medium supplemented with 0.1 mg l−1 6-benzyladenine and 0.5 mg l−1 indole-3-acetic acid. Under these conditions, a high percentage (75–87 %) of cultures remained viable after 18 months in cold cabinets. The stored material was successfully recovered and multiplied normally in the same medium, showing good growth and developing into normal shoots that were morphologically similar to those of non-stored controls. At the histological level, the main change observed was the accumulation of starch granules in cells of the shoot apex, as well as in cells located close to the vascular bundles, after 3 months of cold storage. As the duration of cold storage increased, the number and size of the starch granules decreased but cell plasmolysis and the content of lipid droplets increased. Cold damage was generalized after 24 months at 4 °C. This study provides new insights into the changes occurring in A. glutinosa during cold storage.
Tài liệu tham khảo
Ashmore SE (1997) Status report on the development and application of in vitro techniques for the conservation and use of plant genetic resources. International Plant Genetic Resources Institute, Rome, Italy
Barraco G, Sylvestre I, Collin M, Escoute J, Lartaud M, Verdeil J-L, Engelmann F (2013) Hystocitological analysis of yam (Dioscorea alata) shoot tips cryopreserved by encapsulation-dehydration. Protoplasma 251:177–189
Blakesley D, Pask N, Henshaw GG, Fay MF (1996) Biotechnology and the conservation of forest genetic resources: in vitro strategies and cryopreservation. Plant Growth Regul 20:11–16
Brasier CM, Kirk SA, Delcan J, Cooke DL, Jung T, Man in`tVeld M (2004) Phytophthora alni sp nova and its variants: designation of a group of emerging heteroploid hybrid pathogens. Mycol Res 108:1172–1184
Capuana M, Di Lonardo S (2013) In vitro conservation of chestnut (Castanea sativa) by slow growth. In Vitro Cell Dev Biol Plant 49:605–610
Chmielarz P (2010) Cryopreservation of orthodox seeds of Alnus glutinosa. Cryo Letters 31:139–146
Cree L (2006) Phytophthora alni. EXFOR Database. http://www.spfnic.fs.fed.us/exfor/data/pestreports.cfm?pestidval=28&langdisplay=english
Corredoira E, Valladares S, Martínez T, Couselo JL, San José MC, Ballester A, Vieitez AM (2011) Conservación de germoplasma en especies leñosas con técnicas de cultivo in vitro y almacenamiento en frío. Span J Rural Dev 1:15–24
Eberharter A, Becker PB (2002) Histone acetylation: a switch between repressive and permissive chromatin. EMBO Rep 3:224–229
Engelmann F (1991) In vitro conservation of tropical plant germplasm-a review. Euphytica 57:227–243
Engelmann F (1997) In vitro conservation methods. In: Callow JA, Ford-Lloyd BV, Newburry HJ (eds) Biotechnology and plant genetic resources. Conservation and use. Wallingford pp 119–161
Engelmann F (1999) Management of field and in vitro germplasm collections. In: Proceedings of a Consulting Meeting-15-20 January 1996, CIAT, Cali, Colombia. Int Plant Genet Resour Inst, Rome
Engelmann F (2011) Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cell Dev Biol-Plant 47:5–16
Fulton DC, Stettler M, Mettler T, Vaughan CK, Li J, Francisco P, Gil M, Reinhold H, Eicke S, Messerli G, Dorken G, Halliday K, Smith AM, Smith SM, Zeeman SC (2008) Beta-AMYLASE4, a noncatalytic protein required for starch breakdown, acts upstream of three active beta-amylases in Arabidopsis chloroplasts. Plant Cell 20:1040–1058
Garbero M, Andrade A, Reinoso H, Fernández B, Cuesta C, Granda V, Escudero C, Abdala G, Pedranzani H (2012) Differential effect of short-term cold stress on growth, anatomy, and hormone levels in cold-sensitive versus –resistant cultivars of Digitaria eriantha. Acta Phsiol Plant 34:2079–2091
Hansen J, Kristiansen K (1997) Short-term in vitro storage of Miscanthus x ogiformis Honda ‘Giganteus’ as affected by medium composition, temperature, and photon flux density. Plant Cell Tiss Organ Cult 49:161–169
Janeiro LV, Vieitez AM, Ballester A (1995) Cold storage of in vitro cultures of wild cherry, chestnut and oak. Ann Sci For 52:287–293
Jarillo JA, Pineiro M, Cubas P, Martínez-Zapater M (2009) Chromatin remodeling in plant development. Int J Dev Biol 53:1581–1596
Jensen WA (1962) Botanical histochemistry. In: Freeman H (ed), San Francisco
Jutsuyama Y, Susuki T, Harada T, Fujikawa S (2002) Sucrose incubation increases freezing tolerance of asparagus (Asparagus officinalis L.) embryogenic cell suspensions. Cryo Letters 29:145–156
Kaplan F, Guy CL (2004) Beta-amylase induction and the protective role of maltose during temperature shock. Plant Physiol 135:1674–1684
Kouboris G, Vasilakakis M (2006) Improvement of in vitro propagation of apricot cultivar ‘Bebecou’. Plant Cell Tiss Organ Cult 85:173–180
Kovalchuk I, Lyudvikova Y, Volgina M, Reed BM (2009) Medium, container and genotype all influence in vitro cold storage of apple germplasm. Plant Cell Tiss Organ Cult 96:127–136
Lambardi M, De Carlo A (2003) Application of tissue culture to the germplasm conservation of temperate broad-leaf trees. In: Jain SM, Ishii K (eds) Micropropagation of woody trees and fruits. Kluwer Academic Publishers, Dordrecht, pp 815–840
Lata H, Moraes RM, Bertoni B, Pereira AMS (2010) In vitro germplasm conservation of Podophyllum peltatum L. under slow growth conditions. In Vitro Cell Dev Biol-Plant 46:22–27
Li CY, Yang YQ, Junttila O, Palva ET (2007) Sex-related differences in leaf morphological and physiological responses in Hippophae rhamnoides along an altitudinal gradient. Tree Physiol 27:399–406
Li TA, Xu SL, Oses-Prieto JA, Putil S, Xu P, Wang RJ, Li KH, Maltby DA, An LH, Burlingame AL, Deng ZP, Wang ZY (2011) Proteomics analysis reveals post-translational mechanisms for cold-induced metabolic changes in Arabidopsis. Mol Plant 4:361–374
Lloyd G, McCown B (1980) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip cultures. Comb Proc Int Soc 30:421–427
Lukoseviciute V, Rugienius R, Staniene G, Blazyte A, Gelvonauskiene D, Bendokas V, Gelvonauskis B, Sasnauskas A, Stanys V, Bobinas C (2012) Low temperature storage of Fragaria sp. and Pyrus sp. genetic resources in vitro. Agriculture 99:125–130
Mamum EA, Alfred S, Cantrill LC, Overall RL, Sutton BG (2006) Effects of chilling on male gametophyte development in rice. Cell Biol Int 30:583–591
Marco-Medina A, Casas JL (2012) Polyamine content during minimal growth storage of Thymus moroderi explants. Biol Plant 56:590–594
O’Brien TP, McCully ME (1981) The study of plant structure principles and selected methods. Termarcarphy Pty Ltd, Melbourne
Orlikowska T (1992) Effect of in vitro storage at 4 °C on survival and proliferation of two apple rootstocks. Plant Cell Tiss Organ Cult 31:1–7
Orlikowska T, Zawadzka M, Kuchrska D, Lahuta LB (2010) The influence of the cooling of donor cultures on the in vitro adventitious regeneration and carbohydrate metabolism of four dwarfing apple rootstocks. Acta Physiol Plant 32:333–340
Ozden-Tokatli Y, Akdemir H, Tilkat E, Onay A (2010) Current status and conservation of Pistacia germplasm. Biotechnol Adv 28:130–141
Ozudogru EA, Kirdok E, Kaya E, Capuana M, De Carlo A, Engelmann F (2010) Medium-term conservation of redwood (Sequoia sempervirens (D. Don) Endl.) in vitro shoots cultures and encapsulated buds. Sci Hortic 127:431–435
Rao NK (2004) Plant genetic resources: advancing conservation and use through biotechnology. Afr J Biotechnol 3:136–145
Reed BM, Paynter CL, DeNoma J, Chang Y (1998) Techniques for medium- and long-term storage of Pyrus L. genetic resources. Plant Genet Resour Newsl 115:1–4
Reed BM, Sarasan V, Kane M, Bunna E, Pence VC (2011) Biodiversity conservation and conservation biotechnology tools. In Vitro Cell Dev Bio-Plant 47:1–4
San José MC, Janeiro LV, Corredoira E (2013) Micropropagation of threatened black alder. Silva Fenn 47:1–12
San José MC, Valladares S, Janeiro LV, Corredoira E (2014) Cryopreservation of in vitro-grown shoot tips of Alnus glutinosa (L.) Gaertn. Acta Physiol Plant 36:109–116
Sarasan V, Cripps R, Ramsay MM, Atherton C, Mcmichen M, Prendergast G, Rowntree JK (2006) Conservation in vitro of threatened plants—progress in the past decade. In Vitro Cell Dev Biol-Plant 42:206–214
Saropulus AS, Drennan DSH (2007) Ultrastructural alterations in mesophyll and bundle chloroplasts of two maize cultivars in response to chilling at high irradiance. Biol Plant 51:690–698
Standardi A, Piccioni E (1998) Recent perspectives on synthetic seed technology using nonembryogenic in vitro-derived explants. Int J Plant Sci 159:968–978
Turner S, Krauss SL, Bunn E, Senaratna T, Dixon K, Tan B, Touchell D (2001) Genetic fidelity and viability of Anigozanthos viridis following tissue culture, cold storage and cryopreservation. Plant Sci 161:1099–1106
UNEP (2002) Global Strategy for Plant Conservation. Decision VI/9. Sixth Ordinary Meeting of the Conference of the Parties to the Convention on Biological Diversity, The Hague, 7–19 April 2002 www.cbd.int/decision/cop/default.shtml?id=7180
Vella NGF, Joss TV, Roberts TH (2012) Chilling-induced ultrastructural changes to mesophyll cells of Arabidopsis grown under short days are almost completely reversible by plant re-warming. Protoplasma 249:1137–1149
Watt MP, Thokoane NL, Mycock D, Blackeway F (2000) In vitro storage of Eucalyptus grandis germplasm under minimal growth conditions. Plant Cell Tiss Organ Cult 61:161–164
Zhang S, Jiang H, Peng S, Korpelainen H, Li C (2011) Sex-related differences in morphological, physiological and ultrastructural responses of Populus cathayana to chilling. J Exp Bot 62:675–686