Simple and extractionless high‐performance liquid chromatographic determination of rosiglitazone in human plasma and application to pharmacokinetics in humans

Biomedical Chromatography - Tập 18 Số 8 - Trang 613-615 - 2004
Kyoung‐Ah Kim1, Ji Young Park1
1Department of Pharmacology, Gachon Medical School, Incheon 405-760, Korea.

Tóm tắt

AbstractA simple and extractionless HPLC method using fluorescence detection was developed for the determination of rosiglitazone in human plasma. After deproteinization using perchloric acid the plasma samples were directly injected onto the HPLC system. The mobile phase was composed of acetonitrile (52%) and 20 mm ammonium acetate (48%, pH 7.5), and analysis was run at a flow rate of 0.2 mL/min with the detector operating at 247 nm for excitation wavelength and at 367 nm for emission wavelength, respectively. The method has a mean recovery of 97%, while the intra‐day and inter‐day precisions were all less than 7%. This method is simple, specific, sensitive and requires only a small plasma volume with short analytical time, and is suitable for the determination of plasma rosiglitazone in routine measurements for pharmacokinetic studies. Copyright © 2004 John Wiley & Sons, Ltd.

Từ khóa


Tài liệu tham khảo

10.2165/00003495-199957060-00007

Cox PJ, 2000, Absorption, disposition, and metabolism of rosiglitazone, a potent thiazolidinedione insulin sensitizer, in humans, Drug Metabolism and Disposition, 28, 772

10.1016/S1570-0232(02)01011-5

Mamidi RN, 2002, HPLC method for the determination of rosiglitazone in human plasma and its application in a clinical pharmacokinetic study, Arzneimittelforschung, 52, 560

10.1002/bmc.272

10.1016/S0378-4347(00)00519-3

Niemi M, 2003, Gemfibrozil considerably increases the plasma concentrations of rosiglitazone, Diabetologia, 46, 1319, 10.1007/s00125-003-1181-x

10.1016/j.clpt.2003.10.003

Thông tin
Thông tin xuất bản

Biomedical Chromatography

Tập 18 Số 8

613-615

Thông tin tác giả