Adya P. Singh1, Seung Gon Wi2, Hunseung Kang1, Gap Chae Chung1, Yoon Soo Kim2
1Agricultural Plant Stress Research Center, Chonnam National University, Gwangju, Republic of Korea
2Department of Forest Products and Technology, Chonnam National University, Gwangju, Republic of Korea
Tóm tắt
We developed a method involving air-drying of a rubber suspension after fixation in glutaraldehyde-tannic acid and postfixation in osmium tetroxide for SEM observation. For TEM immunolabeling the suspension was air-dried after osmium-only fixation. Whereas conventional methods failed to satisfactorily stabilize rubber particles, the methods described here proved successful in preserving their integrity.
