Cải thiện đáng kể độ chính xác của phân tích sự di chuyển của tế bào trong vi phim thời gian thực thông qua việc sử dụng hệ thống theo dõi hoàn toàn tự động
Tóm tắt
Sự di động của tế bào là một tham số quan trọng trong nhiều quá trình sinh lý cũng như bệnh lý. Trong vi phim thời gian thực, việc theo dõi tế bào bằng tay vẫn là phương pháp phổ biến nhất để phân tích hành vi di cư của các quần thể tế bào. Ngoài việc mất nhiều công sức, phương pháp này còn dễ bị ảnh hưởng bởi các lỗi phụ thuộc vào người sử dụng liên quan đến việc chọn lựa các tập con "đại diện" của các tế bào và xác định vị trí tế bào một cách thủ công.
Chúng tôi đã phân tích định lượng các nguồn lỗi này, chứng minh rằng việc theo dõi tế bào bằng tay đối với tế bào ung thư tụy dẫn đến tính toán sai tỷ lệ di cư lên đến 410%. Để cung cấp các phép đo khách quan về tỷ lệ di cư của tế bào, chúng tôi đã sử dụng công nghệ theo dõi đa mục tiêu thường được sử dụng trong các ứng dụng radar để phát triển một hệ thống nhận diện và theo dõi tế bào hoàn toàn tự động phù hợp cho việc sàng lọc thông lượng cao các đoạn video của tế bào sống không nhuộm màu.
Từ khóa
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