Significance of Reactive Oxygen Species and Antioxidants in Defining the Efficacy of Sperm Preparation Techniques
Tóm tắt
The mechanisms responsible for mediating the influence of sperm preparation protocols on human sperm function have been investigated. Techniques that involved the separation of motile spermatozoa prior to centrifugation were found to yield sperm suspensions of highest quality. If the spermatozoa were centrifuged prior to isolation of the motile cells, sperm function was impaired. The detrimental effects of centrifugation were associated with a sudden burst of reactive oxygen species production by a discrete subpopulation of cells (characterized by significantly diminished motility and fertilizing capacity) that could be separated from normal functional spermatozoa on Percoll gradients. If unfractionated sperm suspensions were subjected to centrifugation, the reactive oxygen species generated by this subpopulation impaired the functional competence of normal spermatozoa in the same suspension. Assessment of the ability of the antioxidants, butylated hydroxytoluene, and vitamin E, to curtail the peroxidative damage inflicted by such cells in response to centrifugation revealed a significant improvement of sperm function in the presence of vitamin E.
Từ khóa
Tài liệu tham khảo
Aitken RJ, 1987, Free radicals: recent developments in lipid chemistry experimental pathology and medicine, 335
Biggers JD, 1971, Methods in mammalian embryology, 86
Cruz RI, 1986, A prospective study of intrauterine insemination of processed sperm from men with oligoasthenospermia in superovulated women, Fertil Steril, 46, 673, 10.1016/S0015-0282(16)49647-0
Okuma M., 1970, Studies on lipid peroxides in platelets. 1. Method of assay and effect of storage, J Lab Clin Med, 75, 283
SjöblomP. QvistK. SöderlundB. WikO. WiklandM.A comparison of the quality of sperm prepared for IVF with hyaluronic acid and centrifugation/swim up procedures.Proceedings of the European Sterility Congress Organization VIII Budapest Hungary 1987. Abstr 74.
World Health Organization., 1987, WHO laboratory manual for the examination of human semen and semen‐cervical mucus interaction