Side‐to‐side linking of myocardial cells in hypertrophic cardiomyopathy: Whole heart microscopic observation with tangential sections

Pathology International - Tập 55 Số 11 - Trang 677-687 - 2005
Hirotake Masuda1, Misa Yamauchi2, Makoto Yoshida1, Masato Takahashi1, Hiroshi Nanjo3, Yasushi Asari4, Akihiro Sugita2
1Division of Cellular and Organ Pathology, Department of Pathology and Immunology,
2Clinical Pathology, Yuri‐Kumiai General Hospital, Yuri, Japan
3Clinical Pathology, Medical Center, Akita University School of Medicine, Akita,
4Clinical Pathology, Okachi‐Kumiai General Hospital, Yuzawa and

Tóm tắt

By cross‐section or longitudinal section, it is difficult to investigate longitudinal features of myocardial cells in the whole heart. Here, introducing the use of tangential sections to obtain longitudinal aspect of myocardial cells in any part of myocardium, the authors evaluated myocardium in the left ventricle in 10 normal hearts and four hearts with hypertrophic cardiomyopathy (HCM). Tangential sections were obtained by peeling the superficial layer of myocardium. After peeling the whole surface, secondary deep layer was peeled. These procedures were repeated more than five times through the wall. Intercalated discs (ICD) were observed immunohistochemically with anti‐N‐cadherin and antidesmoplakin. In normal hearts, myocardial cells were cut longitudinally and ran parallel in tangential sections. They linked end‐to‐end with simple and regular ICD with average lengths of 120–130 µm and average sarcomere numbers of 56–65. In HCM hearts, many myocardial cells were cut almost longitudinally running approximately parallel in tangential sections. Myocardial cells frequently showed side‐to‐side linking characterized by skewed ICD, indistinct ICD counterparts, and longitudinally arranged ICD. Two young HCM hearts had circle‐shaped ICD and vacuole‐like structures highlighted by immunostaining for N‐cadherin, which were actually extracellular structures comparable with irregular side‐to‐side linking. It is considered that side‐to‐side linking of myocardial cells is a characteristic microscopic feature in HCM rather than myocardial disarray.

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Pathology International

Tập 55 Số 11

677-687

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