Serum N‐glycan analysis in breast cancer patients – Relation to tumour biology and clinical outcome

Molecular Oncology - Tập 10 - Trang 59-72 - 2016
Vilde D. Haakensen1,2, Israel Steinfeld3,4, Radka Saldova5, Akram Asadi Shehni5, Ilona Kifer4, Bjørn Naume6, Pauline M. Rudd5, Anne-Lise Børresen-Dale1,2, Zohar Yakhini3,4
1Department of Genetics, Institute for Cancer Research, Oslo University Hospital – The Norwegian Radium Hospital, Oslo, Norway
2The K.G. Jebsen Center for Breast Cancer Research, Institute for Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway
3Department of Computer Science, Technion, Haifa, Israel
4Agilent Laboratories, Agilent Technologies, Tel-Aviv, Israel
5NIBRT GlycoScience Group, National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, Dublin 4, Ireland
6Department of Oncology, Oslo University Hospital – The Norwegian Radium Hospital, Oslo, Norway

Tóm tắt

Glycosylation and related processes play important roles in cancer development and progression, including metastasis. Several studies have shown that N‐glycans have potential diagnostic value as cancer serum biomarkers. We have explored the significance of the abundance of particular serum N‐glycan structures as important features of breast tumour biology by studying the serum glycome and tumour transcriptome (mRNA and miRNA) of 104 breast cancer patients. Integration of these types of molecular data allows us to study the relationship between serum glycans and transcripts representing functional pathways, such as metabolic pathways or DNA damage response. We identified tri antennary trigalactosylated trisialylated glycans in serum as being associated with lower levels of tumour transcripts involved in focal adhesion and integrin‐mediated cell adhesion. These glycan structures were also linked to poor prognosis in patients with ER negative tumours. High abundance of simple monoantennary glycan structures were associated with increased survival, particularly in the basal‐like subgroup. The presence of circulating tumour cells was found to be significantly associated with several serum glycome structures like bi and triantennary, di‐ and trigalactosylated, di‐ and trisialylated. The link between tumour miRNA expression levels and N‐glycan production is also examined.

Tài liệu tham khảo

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Molecular Oncology

Tập 10

59-72

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