Sequence of psi, a gene on the symbiotic plasmid of Rhizobium phaseoli which inhibits exopolysaccharide synthesis and nodulation and demonstration that its transcription is inhibited by psr, another gene on the symbiotic plasmid

Bagdasarian M, Lurz R, Ruckert B, Franklin FCH, Bagdasarian MM, Frey J, Timmis KN (1981) Specific-purpose plasmid cloning vectors. II Broad host-range high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas. Gene 16:237–247 Beringer JE (1974) R factor transfer in Rhizobium leguminosarum. J Gen Microbiol 83:188–198 Beringer JE, Beynon JL, Buchanan-Wollaston AV, Hirsch PR, Johnston AWB (1978) Introduction of the drug resistance transposon Tn5 into Rhizobium. Nature 276:633–634 Borthakur D, Downie JA, Johnston AWB, Lamb JW (1985) psi, a plasmid-linked Rhizobium phaseoli gene that inhibits exopolysaccharide production and which is required for symbiotic nitrogen fixation. Mol Gen Genet 200:278–282 Buchanan-Wollaston AV, Beringer JE, Brewin NJ, Hirsch PR, Johnston AWB (1980) Isolation of symbiotically defective mutants of Rhizobium leguminosarum by insertion of Tn5 into a transmissible plasmid. Mol Gen Genet 178:185–190 Ditta G, Schmidhauser T, Yakobson E, Lu P, Liang X-W, Finlay DR, Helinski DR (1985) Plasmids related to the broad host range vector, pRK230, useful for gene cloning and for monitoring gene expression. Plasmid 13:149–153 Downie JA, Hombrecher G, Ma Q-S, Knight CD, Wells B, Johnston AWB (1983a) Cloned nodulation genes of Rhizobium leguminosarum determine host range specificity. Mol Gen Genet 190:359–365 Downie JA, Ma Q-S, Knight CD, Hombrecher G, Johnston AWB (1983b) Cloning of the symbiotic region of Rhizobium leguminosarum; the nodulation genes are between the nitrogenase genes and a nifA-like gene. EMBO J 2:347–352 Friedman AM, Long SR, Brown SE, Buikema WJ, Ausubel FM (1982) Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. Gene 18:289–296 Henikoff S (1984) Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359 Johnston AWB, Beynon JL, Buchanan-Wollaston AV, Setchell SM, Hirsch PR, Beringer JE (1978) High frequency transfer of nodulation ability between strains and species of Rhizobium. Nature 276:634–636 Johnston AWB, Hombrecher G, Brewin NJ, Cooper MC (1982) Two transmissible plasmids in Rhizobium leguminosarum strain 300. J Gen Microbiol 128:85–93 Kyte J, Doolittle RF (1982) A simple method for displaying the hydropathic charecter of a protein. J Mol Biol 157:105–132 Lamb JW, Hombrecher G, Johnston AWB (1982) Plasmid-determined nodulation and nitrogen fixation abilities in Rhizobium phaseoli. Mol Gen Genet 86:449–452 Lamb JW, Downie JA, Johnston AWB (1985) Cloning of the nodulation (nod) genes of Rhizobium phaseoli and their homology to R. leguminosarum nod genes. Gene 4:235–241 Long SR (1983) Genetics of Rhizobium nodulation. In: Kosuge T, Nester EW (eds) Plant microbe interactions; Vol. 1 Molecular and genetic perspectives. MacMillan, New York Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, New York Rossen L, Shearman CA, Johnston AWB, Downie JA (1985) The nodD gene of Rhizobium leguminosorum is autoregulatory and in the presence of plant root exudate induces the nodA, B, C genes. EMBO J 3:3369–3373 Ruvkun GB, Ausubel FM (1981) A general method for site-directed mutagenesis in procaryotes. Nature 289:85–88 Sanger F, Coulson AR, Barrel BG, Smith AJH, Roe BA (1980) Cloning single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol 143:161–178 Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467 Schofield PR, Watson JM (1985) Conservation of nif- and speices-specific domains within repeated promoter sequences from fast-growing Rhizobium speices. Nucleic Acids Res 13:3407–3418 Scott KF (1986) Conserved nodulation genes from the non-legume symbiont Bradyrhizobium sp (Parasponia). Nucleic Acids Res 14:2905–2919 Stachell SE, An D, Flores C, Nester EW (1985) A Tn3 lacZ transposon for the random generation fo β-galactosidase gene fusions: aplication to the analysis of gene expression in Agrobacterium. EMBO J 4:891–898 Staden R (1984) Measurements of the effects that coding for a protein has a DNA sequence and their use for finding genes. Nucleic Acids Res 12:551–567 Tully RE, Terry ME (1985) Decreased exopolysaccharide synthesis by anaerobic and symbiotic cells of Bradyrhizobium japonicum. Plant Physiol 79:445–450 Wood WB (1966) Host specificity of DNA produced by E. coli mutations affecting the restriction and modification of DNA. J Mol Biol 16:118–133