Separation and determination of five major opium alkaloids with mixed mode of hydrophilic/cation‐exchange monolith by pressurized capillary electrochromatography

Journal of Separation Science - Tập 30 Số 17 - Trang 3011-3017 - 2007
Xucong Lin1, Jiabin Wang1, Liyu Li1, Xiaochun Wang1, Lu Huang1, Zenghong Xie
1Department of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R. China. Fax: 86‐591‐87893229

Tóm tắt

AbstractA method for the separation and determination of five major opium alkaloids (narcotine, papaverine, thebaine, codeine, and morphine) in pericarpium papaveris by pressurized CEC (pCEC) with monolithic column has been developed. Under the optimum condition, linear calibration ranges of narcotine, papaverine, thebaine, codeine, and morphine were obtained as 2–85, 2–85, 5–75, 10–65, and 10–65 μg/mL, respectively. LODs of these analytes were 1.5–6.0 μg/mL. The RSD (n = 7) of the migration time and peak area were 1.94–5.24 and 4.05–8.21%, respectively. The proposed method was successfully applied to the analysis of pericarpium papaveris samples. Average recoveries of 79.0–95.9% at different fortified levels of alkaloids were achieved with RSD less than 4.6%. Meanwhile, the mechanism of the separation of the alkaloids on the monolithic column was also discussed. The result showed that the separation of alkaloids was mainly based on the mixed mode of hydrophilic interaction (HI) and cation exchange.

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Journal of Separation Science

Tập 30 Số 17

3011-3017

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