Nội dung được dịch bởi AI, chỉ mang tính chất tham khảo
Lựa chọn phôi nang đủ tiêu chuẩn cho việc chuyển giao bằng cách kết hợp giám sát thời gian thực và xét nghiệm CGH mảng cho bệnh nhân trải qua sàng lọc di truyền trước cấy ghép: một nghiên cứu triển vọng với các noãn chị em
Tóm tắt
Những tiến bộ gần đây trong việc giám sát thời gian thực trong điều trị IVF đã cung cấp các dấu hiệu morhokinetic mới cho khả năng phát triển của phôi. Tuy nhiên, còn rất ít thông tin về mối quan hệ giữa các tham số morhokinetic, thành phần nhiễm sắc thể và khả năng cấy ghép. Do đó, nghiên cứu này nhằm điều tra tác động của việc lựa chọn các phôi nang đủ điều kiện để chuyển giao bằng cách kết hợp giám sát thời gian thực và kiểm tra CGH mảng đến kết quả thai kỳ và cấy ghép cho bệnh nhân trải qua sàng lọc di truyền trước cấy ghép (PGS). Tổng cộng có 1163 noãn kỳ II (MII) đã được thu thập từ 138 bệnh nhân PGS với độ tuổi trung bình là 36,6 ± 2,4 năm. Các noãn MII chị em này sau đó đã được ngẫu nhiên chia thành hai nhóm sau khi ICSI: 1) Nhóm A, noãn (n = 582) được nuôi cấy trong hệ thống giám sát thời gian thực và 2) Nhóm B, noãn (n = 581) được nuôi cấy trong lò ấp thông thường. Đối với cả hai nhóm, khuếch đại toàn bộ gen và xét nghiệm CGH mảng được thực hiện sau khi sinh thiết tropectoderm vào ngày thứ 5. Một đến hai phôi nang euploid trong các tham số morhokinetic dự đoán nhất (Nhóm A) hoặc với loại hình thái tốt nhất có sẵn (Nhóm B) đã được chọn để chuyển giao cho từng bệnh nhân vào ngày thứ 6. Tỷ lệ thai kỳ liên tục và tỷ lệ cấy ghép đã được so sánh giữa hai nhóm. Có sự khác biệt đáng kể về tỷ lệ thai kỳ lâm sàng giữa Nhóm A và Nhóm B (71,1% so với 45,9%, tương ứng, p = 0,037). Tỷ lệ cấy ghép được quan sát trên mỗi lần chuyển phôi cũng tăng đáng kể ở Nhóm A so với Nhóm B (66,2% so với 42,4%, tương ứng, p = 0,011). Hơn nữa, một sự gia tăng đáng kể trong tỷ lệ thai kỳ liên tục cũng được quan sát thấy ở Nhóm A so với Nhóm B (68,9% so với 40,5%, tương ứng, p = 0,019). Tuy nhiên, không có sự khác biệt đáng kể về tỷ lệ sảy thai giữa hệ thống giám sát thời gian thực và lò ấp thông thường (3,1% so với 11,8%, tương ứng, p = 0,273). Nghiên cứu này là nghiên cứu triển vọng đầu tiên sử dụng các noãn chị em để đánh giá hiệu quả của việc lựa chọn phôi nang đủ tiêu chuẩn cho việc chuyển giao bằng cách kết hợp giám sát thời gian thực và kiểm tra CGH mảng cho bệnh nhân PGS. Dữ liệu của chúng tôi rõ ràng cho thấy rằng sự kết hợp của hai công nghệ tiên tiến này để chọn ra các phôi nang đủ tiêu chuẩn cho việc chuyển giao dẫn đến tỷ lệ cấy ghép và tỷ lệ thai kỳ liên tục được cải thiện cho bệnh nhân PGS.
Từ khóa
#IVF #phôi nang #giám sát thời gian thực #xét nghiệm CGH mảng #sàng lọc di truyền trước cấy ghép #noãn chị emTài liệu tham khảo
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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1755-8794/7/38/prepub