Selection of a high and stable pigment-producing strain in cultured Euphorbia millii cells
Tóm tắt
A high pigment-producing strain of cultured Euphorbia millii cells was isolated by clonal selection. The pigment obtained was red and consisted mainly of anthocyanin. The amount of this pigment obtained after 24 selections was seven times that found in the original cells. Statistical and cell-pedigree analyses proved that this cell strain has stable productivity for this red pigment.
Tài liệu tham khảo
Matsumoto, T.; Ikeda, T.; Kanno, N.; Kisaki, T.; Noguchi, M. (1980): Selection of high ubiquinone 10-producing strains of tobacco cultured cells by cell cloning technique. Agric. Biol. Chem. 44, 967–969
Murashige, T.; Skoog, F. (1962): A revised medium for rapid growth and bio assays with tobacco tissue culture. Physiol. Plant. 15, 473–497
Ogino, T.; Hiraoka, N.; Tabata, M. (1978): Selection of high nicotine-producing cell lines of tobacco callus by single-cell cloning. Phytochemistry 17, 1907–1910
Yamada, Y.; Watanabe, K. (1980): Selection of high vitamin B6 producing strains in cultured green cells. Agric. Biol. Chem. 44, 2683–2687
Yamamoto, Y.; Mizuguchi, R.; Yamada, Y. (1981): Chemical constituents of cultured cells of Euphorbia tirucalli and E. millii. Plant Cell Rep. (in press)
Zenk, M.H.; El-shagi, H.; Arens, H.; Stöckgt, J.; Weiber, E.W.; Bues, B. (1977): Formation of the indole alkaloids serpentine and ajmalicine in cell suspension cultures of Catharanthus roseus. In: Plant Tissue Culture and its Biotechnological Association (eds. Barz, W.; Reinhard, E.; Zenk, M.H.), pp. 27–43. Berlin, Heidelberg, New York: Springer