Selection and validation of a set of reliable reference genes for quantitative RT-PCR studies in the brain of the Cephalopod Mollusc Octopus vulgaris
Tóm tắt
Từ khóa
Tài liệu tham khảo
Huggett J, Dheda K, Bustin S, Zumla A: Real-time RT-PCR normalisation; strategies and considerations. Genes Immun. 2005, 6: 279-284. 10.1038/sj.gene.6364190
Radonic A, Thulke S, Mackay IM, Landt O, Siegert W, Nitsche A: Guideline to reference gene selection for quantitative real-time PCR. Biochem Biophys Res Commun. 2004, 313: 856-862. 10.1016/j.bbrc.2003.11.177
Skern R, Frost P, Nilsen F: Relative transcript quantification by quantitative PCR: roughly right or precisely wrong?. BMC Mol Biol. 2005 Apr 26;6(1):10. 2005, 6 (1): 10- 10.1186/1471-2199-6-10
Thellin O, Zorzi W, Lakaye B, De Borman B, Coumans B, Hennen G, Grisar T, Igout A, Heinen E: Housekeeping genes as internal standards: use and limits. J Biotechnol. 1999, 75: 291-295. 10.1016/S0168-1656(99)00163-7
Dheda K, Huggett JF, Bustin SA, Johnson MA, Rook G, Zumla A: Validation of housekeeping genes for normalizing RNA expression in real-time PCR. Biotechniques. 2004, 37: 112-114.
Dheda K, Huggett JF, Chang JS, Kim LU, Bustin SA, Johnson MA, Rook GA, Zumla A: The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization. Anal Biochem. 2005, 344: 141-143. 10.1016/j.ab.2005.05.022
Jain M, Nijhawan A, Tyagi AK, Khurana JP: Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochem Biophys Res Commun. 2006, 345: 646-651. 10.1016/j.bbrc.2006.04.140
Liu DW, Chen ST, Liu HP: Choice of endogenous control for gene expression in nonsmall cell lung cancer. Eur Respir J. 2005, 26: 1002-1008. 10.1183/09031936.05.00050205
Mamo S, Gal AB, Bodo S, Dinnyes A: Quantitative evaluation and selection of reference genes in mouse oocytes and embryos cultured in vivo and in vitro. BMC Dev Biol. 2007, 7: 14- 10.1186/1471-213X-7-14
Schmittgen TD, Zakrajsek BA: Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR. J Biochem Biophys Methods. 2000, 46: 69-81. 10.1016/S0165-022X(00)00129-9
Pfaffl MW, Horgan GW, Dempfle L: Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res. 2002, 30 (9 ): e36- 10.1093/nar/30.9.e36
Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002, 3 (7): research0034- 10.1186/gb-2002-3-7-research0034
Borrelli L, Fiorito G: Behavioral Analysis of Learning and Memory in Cephalopods. Learning Theory and Behavior. Vol. 1 (Menzel, R., Volume Editor) of Learning and Memory: A Comprehensive Reference, 4 vols. (Byrne, J., Editor). 2008, 605-628.
Hochner B, Shomrat T, Fiorito G: The octopus: a model for a comparative analysis of the evolution of learning and memory mechanisms. Biol Bull. 2006, 210: 308-317. 10.2307/4134567
Young JZ: The Anatomy of the Central Nervous System of Octopus vulgaris. 1971, Claredon Press, Oxford,
Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP: Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations. Biotechnology Letters. 2004, 26: 509-515. 10.1023/B:BILE.0000019559.84305.47
Andersen CL, Jensen JL, Orntoft TF: Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 2004, 64: 5245-5250. 10.1158/0008-5472.CAN-04-0496
Fernandes JM, Mommens M, Hagen O, Babiak I, Solberg C: Selection of suitable reference genes for real-time PCR studies of Atlantic halibut development. Comp Biochem Physiol B Biochem Mol Biol. 2008, 150: 23-32. 10.1016/j.cbpb.2008.01.003
Olsvik PA, Lie KK, Jordal AEO, Nilsen TO, Hordvik I: Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon. BMC Mol Biol. 2005 Nov 17;6:21. 2005, 6: 21-
Zhong Q, Zhang Q, Wang Z, Qi J, Chen Y, Li S, Sun Y, Li C, Lan X: Expression profiling and validatio of potential reference genes during Paralichthylus olivaceus embryogenesis. Mar Biotechnol (NY). 2008, 10 (3): 310-8. 10.1007/s10126-007-9064-7
Toegel S, Huang WW, Piana C, Unger FM, Wirth M, Goldring MB, Gabor F, Viernstein H: Selection of reliable reference genes for qPCR studies on chondroprotective action. BMC Mol Biol. 2007, 8: 13- 10.1186/1471-2199-8-13
Hibbeler S, Scharsack JP, Becker S: Housekeeping genes for quantitative expression studies in the three-spined stickleback Gasterosteus aculeatus. BMC Mol Biol. 2008, 9: 18- 10.1186/1471-2199-9-18
Messenger JB: Neurotransmitters of cephalopods. Invertebrate Neuroscience. 1996, 2: 95-114. 10.1007/BF02214113.
Messenger JB, Nixon M, Ryan KP: Magnesium chloride as an anaesthetic for cephalopods. Comp Biochem Physiol C Comp Pharmacol. 1985, 82: 203-205. 10.1016/0742-8413(85)90230-0.
Rozen S, Skaletsky HJ: Primer3 on the WWW for general users and for biologist programmers. Bioinformatics Methods and Protocols: Methods in Molecular Biology. Edited by: Krawetz S, Misener S. 2000, 365-386. Totowa, NJ: Humana Press