Scopoletin Supplementation Ameliorates Steatosis and Inflammation in Diabetic Mice

Phytotherapy Research - Tập 31 Số 11 - Trang 1795-1804 - 2017
Ra‐Yeong Choi1, Ju Ri Ham1, Hae-in Lee2, Hyun Wook Cho3, Myung‐Sook Choi4,5, Seok‐Kyu Park1, Jin Lee1, Myung‐Joo Kim6, Kwon‐Il Seo7, Mi‐Kyung Lee1
1Department of Food and Nutrition, Sunchon National University, Suncheon, 57922 Korea
2Mokpo Marin Food-Industry Research Center, Mokpo, 58621 Korea
3Department of Biology, Sunchon National University, Suncheon, 57922 Korea
4Center for Food and Nutritional Genomic Research, Kyungpook National University, Daegu, 41566 Korea
5Department of Food Science and Nutrition, Kyungpook National University, Daegu, 41566 Korea
6Department of Bakery and Barista, Suseong College, Daegu, 42078 Korea
7Department of Biotechnology, Dong-A University, Busan, 49315 Korea

Tóm tắt

Scopoletin is a bioactive component in many edible plants and fruits. This study investigated the effects of scopoletin on hepatic steatosis and inflammation in a high‐fat diet fed type 1 diabetic mice by comparison with metformin. Scopoletin (0.01%, w/w) or metformin (0.5%, w/w) was provided with a high‐fat diet to streptozotocin‐induced diabetic mice for 11 weeks. Both scopoletin and metformin lowered blood glucose and HbA1c, serum ALT, TNF‐α and IL‐6 levels, glucose intolerance, and hepatic lipid accumulation compared with the diabetic control group. Scopoletin or metformin down‐regulated hepatic gene expression of triglyceride (Pparg, Plpp2, and Dgat2) and cholesterol (Hmgcr) synthesis as well as inflammation (Tlr4, Myd88, Nfkb1, Tnfa, and Il6), while it up‐regulated Cyp7a1 gene. Hepatic PPARγ and DGAT2 protein levels were also down‐regulated in scopoletin or metformin group compared with the control group. Scopoletin or metformin also inhibited hepatic fatty acid synthase and phosphatidate phosphohydrolase activities. These results suggest that scopoletin protects against diabetes‐induced steatosis and inflammation by inhibiting lipid biosynthesis and TLR4‐MyD88 pathways. Copyright © 2017 John Wiley & Sons, Ltd.

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Phytotherapy Research

Tập 31 Số 11

1795-1804

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