Sampling and pyrosequencing methods for characterizing bacterial communities in the human gut using 16S sequence tags

Gary D. Wu1, James D. Lewis1, Christian Hoffmann2, Ying Yu Chen1, Rob Knight3, Kyle Bittinger2, Jennifer Hwang2, Jun Chen4, Ronald W. Berkowsky1, Lisa Nessel4, Hongzhe Li4, Frederic D. Bushman2
1Division of Gastroenterology, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104-6076, USA
2Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6076 USA
3Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO, 80309-0215, USA
4Center for Clinical Epidemiology and Biostatistics, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104-6076, USA

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