Salivary Amylase Promotes Adhesion of Oral Streptococci to Hydroxyapatite

SAGE Publications - Tập 74 Số 7 - Trang 1360-1366 - 1995
Frank A. Scannapieco1, Guillermo Torres2, Michael J. Levine1
1Department of Oral Biology and Dental Research Institute, School of Dental Medicine, State University of New York at Buffalo, Buffalo, New York 14214
2Department of General Dentistry, Eastman Dental Center, Rochester, New York 14620

Tóm tắt

Recent studies have demonstrated that several species of oral streptococci, such as Streptococcus gordonii, bind soluble salivary a-amylase. The goal of the present study was to determine if amylase immobilized onto a surface such as hydroxyapatite can serve as an adhesion receptor for S. gordonii. Initially, human parotid saliva was fractionated on Bio-Gel P60, and fractions were screened for their ability to promote adhesion of S. gordonii to hydroxyapatite. Fractions containing a-amylase and proline-rich proteins promoted the adhesion of [3H]-labeled S. gordonii to hydroxyapatite. Similar findings were obtained with purified amylase and acidic proline-rich protein 1 (PRP1). Incubation of S. gordonii G9B in the presence of starch and maltotriose increased the binding of this strain to amylase-coated hydroxyapatite, while the adhesion of S. sanguis 10556 to amylase-coated hydroxyapatite was not affected by these saccharides. These results suggest that amylase may serve as a hydroxyapatite pellicle receptor for amylase-binding streptococci. Furthermore, starch and starch metabolites may enhance the adhesion of amylase-binding streptococci to amylase in dental pellicles to augment the formation of dental plaque.

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Tài liệu tham khảo

Al-Hashimi I, Levine MJ (1989). Characterization of in vivo salivary-derived enamel pellicle. Arch Oral Biol 34:289-295. Bergey EJ, Levine MJ, Reddy MS, Bradway SD, Al-Hashimi I (1986). Use of the photoaffinity cross-linking agent N-hydroxysuccinimidyl-4-azidosalicylic acid to characterize salivary glycoprotein bacterial interactions. Biochem J 234:43-48.

Birkhed D., 1978, Scand J Dent Res, 86, 248

10.1042/bj2840557

10.1128/IAI.60.3.1008-1017.1992

10.1128/IAI.18.2.514-523.1977

10.1128/IAI.19.3.846-853.1978

10.1128/IAI.57.10.3003-3008.1989

10.1016/0003-9969(84)90122-5

10.1016/0003-9969(83)90003-1

10.1177/00220345900690110701

10.1111/j.1574-6968.1990.tb03995.x

10.1016/0022-3913(92)90387-P

10.1177/00220345890680050101

Gibbons RJ, 1988, Human salivary acidic proline-rich

10.1128/IAI.56.2.439-445.1988

Gibbons RJ, Hay DI (1989). Adsorbed salivary acidic proline-rich proteins contribute to the adhesion of Streptococcus mutans JPB to apatitic surfaces. J Dent Res 68:1303-1307. Gibbons RJ, Moreno EC, Spinell DM (1976). Model delineating the effects of a salivary pellicle on the adsorption of Streptococcus miteor onto hydroxyapatite. Infect Immun 14:1109-1112.

Gibbons RJ, Etherden I., Peros W. (1985). Aspects of the attachment of oral streptococci to experimental pellicles. In: Molecular basis of oral microbial adhesion. Mergenhagen S, Rosan B, editors. Washington, DC: American Society for Microbiology, pp. 77-84.

10.1128/IAI.56.11.2990-2993.1988

10.1128/IAI.59.9.2948-2954.1991

10.1016/S0021-9258(19)47381-X

10.1159/000259553

10.1042/bj2550015

Johnsson M., 1990, In: Material synthesis utilizing biological processes. Rieke PC, Calvert PD, editors. Mater Res Soc Proc, 174, 81

10.1128/JCM.28.11.2576-2577.1990

10.1016/0003-9969(80)90046-1

10.1128/IAI.60.9.3878-3884.1992

10.1128/IAI.26.3.1104-1110.1979

10.1093/oxfordjournals.jbchem.a134659

Nyvad B., 1987, Scand J Dent Res, 95, 369

10.1159/000261281

10.1111/j.1600-0714.1973.tb01675.x

0rstavik D., 1974, Scand J Dent Res, 82, 202

Rosan B., Eifert R., Golub E. (1985). Bacterial surfaces, salivary pellicles and plaque formation. In: Molecular basis of oral microbial adhesion. Mergenhagen S, Rosan B, editors. Washington, DC: American Society for Microbiology, pp. 69-76.

Scannapieco FA (1995). Role of saliva-bacterium interactions in oral microbial ecology. Crit Rev Oral Biol Med 5:203-248. Scannapieco FA, Bergey EJ, Reddy MS, Levine MJ (1989). Characterization of salivary a-amylase binding to Streptococcus sanguis. Infect Immun 57:2853-2863. Scannapieco FA, Bhandary K, Ramasubbu N, Levine MJ (1990). Structural relationship between the enzymatic and streptococcal binding sites of human salivary a-amylase. Biochem Biophys Res Commun 173:1109-1115.

Scannapieco FA, 1992, Characterization of an amylase-binding component from

Scannapieco FA, 1993, Infect Immun, 60, 4726, 10.1128/iai.60.11.4726-4733.1992

10.1177/00220345940730100701

Stromberg N, Boren T, Carlen A, Olsson J (1992). Salivary receptors for GalNAcß-sensitive adherence of Actinomyces spp.: evidence for heterogeneous GalNAcß and proline-rich protein receptor properties. Infect Immun 60:3278-3286. Syed SA, Loesche WJ (1978). Bacteriology of human gingivitis: Effect of plaque age. Infect Immun 21:821-829.

Tellefson LM, Germaine GR (1986). Adherence of Streptococcus sanguis to hydroxyapatite coated with lysozyme and lysozyme-supplemented saliva. Infect Immun 51:750-759. Terleckyj B, Willet NP, Schockman GD (1975). Growth of several cariogenic strains of oral streptococci in a chemically defined medium. Infect Immun 11:649-655. Torres GI, Reddy MS, Scannapieco FA, Levine MJ (1993). Low molecular-weight salivary mucin promotes adhesion of Streptococcus gordonii to hydroxyapatite (abstract). J Dent Res 72:363.

10.1111/j.1399-302X.1992.tb00021.x

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SAGE Publications

Tập 74 Số 7

1360-1366

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