SYNTHESIS OF PEPTIDES BY FRAGMENT CONDENSATION ON A SOLID SUPPORT.

Wiley - Tập 7 Số 4 - Trang 307-312 - 1975
Ulf Ragnarsson1, Sune Karlsson1, Ulla Hamberg1
1Biokemiska Institutionen, Uppsala Universitet, Uppsala, Sweden and Biokemiska Institutionen, Helsingfors Universitet, Helsingfors, Finland

Tóm tắt

Two tripeptides and one dipeptide, protected except at the carboxyl ends, have been prepared in solution and used as intermediates in a new synthesis of bradykinin on a solid support. Condensation of the two tripeptides to the resin was effected in satisfactory yield with dicyclohexylcarbodiimide plus N‐hydroxysuccinimide. The partial epimerization that might occur by such an approach was explicitly verified to be without practical importance in this case. The crude product contained only traces of impurities and yielded, after purification, bradykinin of high purity. Both the crude and purified bradykinin exhibited full biological activity.

Từ khóa


Tài liệu tham khảo

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Thông tin
Thông tin xuất bản

Wiley

Tập 7 Số 4

307-312

Thông tin tác giả