Thiếu hụt SWAP-70 gây ra sự hình thành tế bào plasma có ái lực cao mặc dù hình thành trung tâm mầm bị suy yếu

Blood - Tập 111 - Trang 2714-2724 - 2008
Laurence Quemeneur1, Veronique Angeli1, Michael Chopin2, Rolf Jessberger1,2
1Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY; and
2Institute of Physiological Chemistry, Dresden University of Technology , Dresden , Germany

Tóm tắt

Các trung tâm mầm (GCs) là các cấu trúc mô lympho trung tâm trong việc tạo ra các tế bào B sản sinh kháng thể có độ bền cao và ái lực lớn. Tuy nhiên, việc kích thích, duy trì và điều hòa các GCs chưa được hiểu rõ. Protein SWAP-70, liên kết với F-actin và tương tác với Rac, được biểu hiện mạnh mẽ ở các tế bào B hoạt hóa như ở các nang B. Công trình mới đây gợi ý rằng SWAP-70 tham gia vào việc kích hoạt tế bào B, di chuyển và định cư. Do đó, chúng tôi đã điều tra vai trò của SWAP-70 trong phản ứng miễn dịch phụ thuộc vào T, trong việc hình thành GCs, và trong sự phân hóa thành các tế bào plasma và tế bào B nhớ. So với chuột wt, chuột SWAP-70−/− được miễn dịch với tế bào máu đỏ cừu (SRBC) hoặc NP-KLH cho thấy số lượng GCs và tế bào B đặc hiệu GC giảm mạnh. Tuy nhiên, các tế bào B cụ thể NP ở chuột SWAP-70−/− tích tụ bên ngoài các nang B, và chuột SWAP-70−/− cho thấy nhiều tế bào plasma hơn trong tủy đỏ và trong tủy xương, cũng như tăng Ig cụ thể NP và tế bào B hình thành kháng thể. Tuy nhiên, phản ứng ký ức bị suy giảm. Do đó, sự thiếu hụt SWAP-70 làm phá vỡ mối liên hệ giữa hình thành GC và sản xuất kháng thể phụ thuộc vào T và tế bào plasma tồn tại lâu dài, gây ra sự hình thành các tế bào plasma có ái lực cao bên ngoài nang, nhưng không hỗ trợ đầy đủ cho phản ứng ký ức.

Từ khóa


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