S1 domain‐containing STF modulates plastid transcription and chloroplast biogenesis in Nicotiana benthamiana

New Phytologist - Tập 193 Số 2 - Trang 349-363 - 2012
Young Ho Jeon1, Hyun‐Ju Jung2, Hunseung Kang2, Youn–Il Park3, Soon Hee Lee1, Hyun‐Sook Pai1
1Department of Systems Biology, Yonsei University, Seoul 120-749, Korea
2Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500–757, Korea
3Department of Biological Science and Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, Korea

Tóm tắt

Summary

In this study, we examined the biochemical and physiological functions of Nicotiana benthamiana S1 domain‐containing Transcription‐Stimulating Factor (STF) using virus‐induced gene silencing (VIGS), cosuppression, and overexpression strategies.

STF : green fluorescent protein (GFP) fusion protein colocalized with sulfite reductase (SiR), a chloroplast nucleoid‐associated protein also present in the stroma. Full‐length STF and its S1 domain preferentially bound to RNA, probably in a sequence‐nonspecific manner.

STF silencing by VIGS or cosuppression resulted in severe leaf yellowing caused by disrupted chloroplast development. STF deficiency significantly perturbed plastid‐encoded multimeric RNA polymerase (PEP)‐dependent transcript accumulation. Chloroplast transcription run‐on assays revealed that the transcription rate of PEP‐dependent plastid genes was reduced in the STF‐silenced leaves. Conversely, the exogenously added recombinant STF protein increased the transcription rate, suggesting a direct role of STF in plastid transcription. Etiolated seedlings of STF cosuppression lines showed defects in the light‐triggered transition from etioplasts to chloroplasts, accompanied by reduced light‐induced expression of plastid‐encoded genes.

These results suggest that STF plays a critical role as an auxiliary factor of the PEP transcription complex in the regulation of plastid transcription and chloroplast biogenesis in higher plants.

Từ khóa


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New Phytologist

Tập 193 Số 2

349-363

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