Rv1818c‐encoded PE_PGRS protein of <i>Mycobacterium tuberculosis</i> is surface exposed and influences bacterial cell structure

Molecular Microbiology - Tập 52 Số 3 - Trang 725-733 - 2004
Giovanni Delogu1,2, Clara Pusceddu1, Alessandra Bua1, Giovanni Fadda3, Michael J. Brennan4, Stefania Zanetti1
1Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy
2Present address: Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo F. Vito, 00168 Roma, Italy.
3Institute of Microbiology, Catholic University of the Sacred Heart, Rome, Italy
4Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD, USA

Tóm tắt

SummaryIdentification of the novel PE multigene family was an unexpected finding of the genomic sequencing of Mycobacterium tuberculosis. Presently, the biological role of the PE and PE_PGRS proteins encoded by this unique family of mycobacterial genes remains unknown. In this report, a representative PE_PGRS gene (Rv1818c/PE_PGRS33) was selected to investigate the role of these proteins. Cell fractionation studies and fluorescence analysis of recombinant strains of Mycobacterium smegmatis and M. tuberculosis expressing green fluorescent protein (GFP)‐tagged proteins indicated that the Rv1818c gene product localized in the mycobacterial cell wall, mostly at the bacterial cell poles, where it is exposed to the extracellular milieu. Further analysis of this PE_PGRS protein showed that the PE domain is necessary for subcellular localization. In addition, the PGRS domain, but not PE, affects bacterial shape and colony morphology when Rv1818c is overexpressed in M. smegmatis and M. tuberculosis. Taken together, the results indicate that PE_PGRS and PE proteins can be associated with the mycobacterial cell wall and influence cellular structure as well as the formation of mycobacterial colonies. Regulated expression of PE genes could have implications for the survival and pathogenesis of mycobacteria within the human host and in other environmental niches.

Từ khóa


Tài liệu tham khảo

10.1046/j.1365-2958.2002.02813.x

10.1111/j.1365-2958.2005.04824.x

10.1128/jb.171.6.3465-3470.1989

10.1016/0923-2508(94)90024-8

10.1016/S0966-842X(02)02335-1

10.1128/IAI.69.12.7326-7333.2001

10.1128/JB.181.2.521-530.1999

10.1016/S0014-5793(99)00536-0

10.1038/31159

10.1016/S0092-8674(03)00421-5

10.1128/IAI.69.9.5606-5611.2001

10.1128/IAI.70.1.292-302.2002

10.1111/j.1365-2958.1995.mmi_17050901.x

10.1128/IAI.67.6.2941-2950.1999

10.1016/S1369-5274(00)00160-0

10.1099/00221287-145-12-3487

10.1128/JB.184.19.5479-5490.2002

10.1128/jb.177.7.1719-1726.1995

10.1046/j.1365-2958.1999.01213.x

10.1016/S1097-2765(00)80250-6

10.1126/science.287.5457.1477

10.1073/pnas.95.4.1523

10.1073/pnas.1231432100

10.1046/j.1365-2958.2002.03162.x

10.1073/pnas.94.9.4721

10.1046/j.1365-2958.2000.01738.x

10.1046/j.1365-2958.2002.03237.x

10.1126/science.288.5470.1436

10.1006/mpat.1996.0046

10.1074/jbc.274.44.31625

10.1038/351456a0

Tyagi A.K., 2000, Molecular Genetics of Mycobacteria, 131

10.1006/mpat.1999.0312

10.1073/pnas.261348198

10.1111/j.1365-313X.1991.00175.x

Thông tin
Thông tin xuất bản

Molecular Microbiology

Tập 52 Số 3

725-733

Thông tin tác giả