Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action

Wiley - Tập 171 Số 3 - Trang 347-368 - 1971
John H. Luft1
1Department of Biological Structure, University of Washington, Seattle, Washington 98105

Tóm tắt

AbstractThe properties of the inorganic dye ruthenium red are presented with emphasis upon its use for electron microscopy of cells and tissues. Although commercial ruthenium red often can be used directly, it always contains various impurities and by‐products. One of these, termed ruthenium violet, can be isolated and is useful by itself. Absorption spectra of the ruthenium dyes and common impurities are given so that an assay is possible for any sample. Convenient fixative recipes containing ruthenium red or violet are provided together with constraints necessary for a reliable reaction to label extracellular acidic mucosubstances. Perfusion was not successful. The specificity of the ruthenium red reaction was evaluated by spot testing with 57 substances, and by titration with chemically defined pectins. The results indicate that ruthenium red, as a hexavalent cation, precipitates a large variety of polyanions by ionic interaction, and that its classical reaction with pectin is typical rather than specific. New data are presented regarding its reaction with phospholipids. For electron microscopy, a further reaction with OsO4 amplifies the feeble electron density, which is the counterpart of its intense optical labeling, to a practical level resulting in strong contrast. An hypothesis is presented for the mechanism underlying this intensification.

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Tài liệu tham khảo

10.1083/jcb.8.2.501

10.1016/S0008-6215(00)82963-8

Avtokratova T. D., 1963, Analytical chemistry of ruthenium

Bauer H., 1933, Mikroskopisch‐chemischer Nachweis von Glykogen und einigen anderen Polysacchariden, Z. mikroskop. anat. Forsch., 33, 143

10.1016/S0022-5320(70)90159-0

10.1007/BF02869919

Brode W. R., 1950, Physical Methods in Chemical Analysis, 193

Casella C., 1942, II Permanganato di Potassio quale ossidante in alcune reazioni istochimiche dei polisaccaridi, Anat. Anz., 93, 289

10.1016/S0074-7696(08)60407-6

Deuel H. J., 1951, The Lipids. Their Chemistry and Biochemistry, Vol. I: Chemistry, 418, 10.5962/bhl.title.6964

Eisen G., 1897, Notes on fixation, stains, the alcohol method, etc., Z. wiss. Mikro., 14, 195

Faaske E., 1965, Über die Anfärbbarkeit saurer Mucopolysaccharide mit Rutheniumrot, Z. wiss. Mikro., 67, 47

Falk H., 1960, Untersuchungen am Caspary‐Streifen, 1063

10.1016/0022-1902(55)80048-6

Fletcher J. M. B. F. Greenfield C. J.Hardy D.ScargillandJ. L.Woodhead(1961) Ruthenium red.J. Chem. Soc. 2000–2006.

Fletcher J. M. W. E. Gardner B. F.Greenfield M. J.HoldowayandM. H.Rand(1968) Magnetic and other studies of ruthenium dioxide and its hydrate.J. Chem. Soc (A) 653–657.

10.1083/jcb.8.3.719

Griffith W. P.(1964) Infrared spectra and structures of osmium oxy‐complexes.J. Chem. Soc. 245–249.

Griffith W. P., 1967, The Chemistry of the Rarer Platinum Metals (Os, Ru, Ir and Rh)

10.1524/zpch.1957.11.1_2.120

Heidenhain M., 1913, Über die Bearbeitung der Sehnen zu Kurszwecken, insbesondere über die Verwendung des Rutheniumrots und der Malloryschen Bindgewebsfärbung, Z. wiss. Mikro., 30, 161

Hotchkiss R. D., 1948, A microchemical reaction resulting in the staining of polysac charide structures in fixed tissue preparations, Arch. Biochem., 16, 131

Jensen W. A., 1962, Botanical Histochemistry. Principles and Practice

Joly A., 1892, Composés ammoniacaux dérivés du sesquichlorure de ruthénium, C. R. Acad. Sci., 115, 1299

10.1080/00268976100100311

10.1039/j19690001477

Luft J. H., 1964, Electron microscopy of cell extraneous coats as revealed by ruthenium red staining, J. Cell Biol., 23, 54A

Luft J. H., 1965, The fine structure of hyaline cartilage matrix following ruthenium red fixation and staining, J. Cell Biol., 27, 61A

Luft J. H., 1966, Ruthenium red and violet. I. Chemistry, purification, methods of use and mechanism of action

10.1002/ar.1091710303

Mangin L., 1890, Sur les réactifs colorants des substances fondamentales de la membrane, C. R. Acad. Sci., 111, 120

Mangin L., 1893, Sur l'emploi du rouge de ruthénium en anatomie végétale, C. R. Acad. Sci., 116, 653

10.1038/158202a0

10.1042/bj0190979

Mills G. A., 1964, Kirk‐Othmer Encyclopedia of Chemical Technology, 534

Morgan G. T. andF. H.Burstall(1936) Researches on residual affinity and co‐ordination. Part XXXVI. The constitution of “ruthenium red.”J. Chem. Soc. 41–45.

Nicolle M., 1893, Propriétés colorantes de l'oxychlorure de ruthénium ammoniacal, Ann. Inst. Pasteur, 7, 331

Pauling L., 1960, The Nature of the Chemical Bond and the Structure of Molecules and Crystals: An Introduction to Modern Structural Chemistry, 172

Persson B. H., 1953, Studies on connective tissue ground substance, Acta soc. medicorum Upsaliensis, 58, 1

10.1007/BF01054291

10.5962/bhl.title.4880

10.1111/j.1469-8137.1924.tb06618.x

10.1007/BF00306150

Reimann B., 1961, Zur Verwendbarkeit von Rutheniumrot als elektronenmikroskopisches Kontrastierungsmittel, Mikroskopie, 16, 224

10.1083/jcb.24.1.39

Schnepf E., 1963, Zur Cytologie und Physiologie pflanzlicher Drüsen 1. Teil. Über den Fangschleim der Insektivoren, Flora (Jena), 153, 1

10.1007/BF01258543

10.1007/BF00306149

10.1083/jcb.6.3.423

Sidgwick N. V., 1950, The Chemical Elements and Their Compounds, 2, 1459

10.2307/2440510

10.1002/ar.1091710304

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Wiley

Tập 171 Số 3

347-368

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