Rotational Coupling of the Transmembrane and Kinase Domains of the Neu Receptor Tyrosine Kinase

Molecular Biology of the Cell - Tập 11 Số 10 - Trang 3589-3599 - 2000
Charlotte A. Bell1, John A. Tynan1, Kristen C. Hart1, April N. Meyer1, Scott C. Robertson1, Daniel J. Donoghue1
1Department of Chemistry and Biochemistry, and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093-0367

Tóm tắt

Ligand binding to receptor tyrosine kinases (RTKs) regulates receptor dimerization and activation of the kinase domain. To examine the role of the transmembrane domain in regulation of RTK activation, we have exploited a simplified transmembrane motif, [VVVEVVV]n, previously shown to activate the Neu receptor. Here we demonstrate rotational linkage of the transmembrane domain with the kinase domain, as evidenced by a periodic activation of Neu as the dimerization motif is shifted across the transmembrane domain. These results indicate that activation requires a specific orientation of the kinase domains with respect to each other. Results obtained with platelet-derived growth factor receptor-β suggest that this rotational linkage of the transmembrane domain to the kinase domain may be a general feature of RTKs. These observations suggest that activating mutations in RTK transmembrane and juxtamembrane domains will be limited to those residues that position the kinase domains in an allowed rotational conformation.

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Tài liệu tham khảo

Bargmann C.I., 1986, Nature, 319, 226, 10.1038/319226a0

Bargmann C.I., 1986, Cell, 45, 649, 10.1016/0092-8674(86)90779-8

Bargmann C.I., 1988, Proc. Natl. Acad. Sci. USA, 85, 5394, 10.1073/pnas.85.15.5394

Bargmann C.I., 1988, EMBO J., 7, 2043, 10.1002/j.1460-2075.1988.tb03044.x

Brandt-Rauf P.W., 1990, Proc. Natl. Acad. Sci. USA, 87, 8660, 10.1073/pnas.87.21.8660

Burke C.L., 1997, Oncogene, 14, 687, 10.1038/sj.onc.1200873

Burke C.L., 1998, Mol. Cell. Biol., 18, 5371, 10.1128/MCB.18.9.5371

Cao H., 1992, EMBO J., 11, 923, 10.1002/j.1460-2075.1992.tb05131.x

Chen C., 1987, Mol. Cell. Biol., 7, 2745, 10.1128/MCB.7.8.2745

Chen L.I., 1997, J. Cell Biol., 137, 619, 10.1083/jcb.137.3.619

D'Avis P.Y., 1998, Cell Growth Differ., 9, 71

Dankort D.L., 1997, Mol. Cell. Biol., 17, 5410, 10.1128/MCB.17.9.5410

Deng T., 1994, Nature, 371, 171, 10.1038/371171a0

Fantl W.J., 1993, Annu. Rev. Biochem., 62, 453, 10.1146/annurev.bi.62.070193.002321

Fazioli F., 1991, Mol. Cell. Biol., 11, 2040, 10.1128/MCB.11.4.2040

Feller S.M., 1994, Trends Biochem. Sci., 19, 453, 10.1016/0968-0004(94)90129-5

Feng G.S., 1994, Trends Genet., 10, 54, 10.1016/0168-9525(94)90149-X

Galvin B.D., 1996, Proc. Natl. Acad. Sci. USA, 93, 7894, 10.1073/pnas.93.15.7894

Gullick W.J., 1992, EMBO J., 11, 43, 10.1002/j.1460-2075.1992.tb05025.x

Heldin C.H., 1995, Cell, 80, 213, 10.1016/0092-8674(95)90404-2

Heldin C.-H., 1996, Cancer Surv., 27, 7

Hirota S., 1998, Science, 279, 577, 10.1126/science.279.5350.577

Hu Q., 1995, Mol. Cell. Biol., 15, 1169, 10.1128/MCB.15.3.1169

Johnson D.L., 1998, Biochemistry, 37, 3699, 10.1021/bi971956y

Kitayama H., 1995, Blood, 85, 790, 10.1182/blood.V85.3.790.bloodjournal853790

Kuriyan J., 1997, Annu. Rev. Biophys. Biomol. Struct., 26, 259, 10.1146/annurev.biophys.26.1.259

Livnah O., 1998, Nat. Struct. Biol., 5, 993, 10.1038/2965

Livnah O., 1996, Science, 273, 464, 10.1126/science.273.5274.464

Livnah O., 1999, Science, 283, 987, 10.1126/science.283.5404.987

Meyers G.A., 1995, Nat. Genet., 11, 462, 10.1038/ng1295-462

Moskaluk C.A., 1999, Oncogene, 18, 1897, 10.1038/sj.onc.1202496

Naski M.C., 1996, Nat. Genet., 13, 233, 10.1038/ng0696-233

Petti L.M., 1998, Oncogene, 16, 843, 10.1038/sj.onc.1201590

Pinkas-Kramarski R., 1996, EMBO J., 15, 2452, 10.1002/j.1460-2075.1996.tb00603.x

Qian X., 1995, Oncogene, 10, 211

Rousseau F., 1994, Nature, 371, 252, 10.1038/371252a0

Santoro M., 1995, Science, 267, 381, 10.1126/science.7824936

Shiang R., 1994, Cell, 78, 335, 10.1016/0092-8674(94)90302-6

Shokat K.M., 1995, Chem. Biol., 2, 509, 10.1016/1074-5521(95)90183-3

Siegel P.M., 1994, Mol. Cell. Biol., 14, 7068, 10.1128/MCB.14.11.7068

Smith S.O., 1996, Nat. Struct. Biol., 3, 252, 10.1038/nsb0396-252

Sorokin A., 1994, J. Biol. Chem., 269, 9752, 10.1016/S0021-9258(17)36947-8

Sternberg M.J.E., 1989, Nature, 339, 587, 10.1038/339587a0

Sternberg M.J.E., 1990, Protein Eng., 3, 245, 10.1093/protein/3.4.245

Ullrich A., 1990, Cell, 61, 203, 10.1016/0092-8674(90)90801-K

Webster M.K., 1996, EMBO J., 15, 520, 10.1002/j.1460-2075.1996.tb00384.x

Webster M.K., 1997, Trends Genet., 13, 178, 10.1016/S0168-9525(97)01131-1

Weiner D.B., 1989, Nature, 339, 230, 10.1038/339230a0

Weiss A., 1998, Cell, 94, 277, 10.1016/S0092-8674(00)81469-5

Weiss F.U., 1997, Curr. Opin. Genet. Dev., 7, 80, 10.1016/S0959-437X(97)80113-X

Williams L.T., 1989, Science, 243, 1564, 10.1126/science.2538922

Xie Y., 1995, Oncogene, 10, 2409

Yarden Y., 1986, Nature, 323, 226, 10.1038/323226a0

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Molecular Biology of the Cell

Tập 11 Số 10

3589-3599

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