Role of miR‐486‐5p in regulating renal cell carcinoma cell proliferation and apoptosis via TGF‐β–activated kinase 1

Journal of Cellular Biochemistry - Tập 120 Số 3 - Trang 2954-2963 - 2019
Yanfa He1, Jianzhen Liu2, Yongjun Wang3, Xiaoli Zhu2, Zhengchao Fan2, Chongbin Li2, Hang Yin2, Ying Liu4
1Cardiac Surgery Department, Hebei Chest Hospital, Shijiazhuang, Hebei, China
2Urology Department, Hebei Chest Hospital, Shijiazhuang, Hebei, China
3Cardiovascular Department, Hebei Chest Hospital, Shijiazhuang, Hebei, China
4The Department of Oncology, Hebei Chest Hospital, Shijiazhuang, Hebei, China

Tóm tắt

AbstractRenal cell carcinoma (RCC) is a common kidney tumor in adults. The role of miR‐486‐5p in RCC is unknown. The aim of our study was to identify new targets regulated by miR‐486‐5p in RCC, to obtain a deeper insight into the network and to better understand the role of these microRNAs and their targets in carcinogenesis of RCC. We performed a series of tests and found consistently lower expression levels of miR‐486‐5p in kidney cancer cells. Restoration of miR‐486‐5p expression in RCC cells could lead to the suppression of cell proliferation and the increase of cell apoptosis. Further studies demonstrated that TGF‐β–activated kinase 1 was a target gene of miR‐486‐5p in kidney cancer cells. It was also shown that C‐C motif chemokine ligand 2 (CCL2) from tumor‐associated macrophages downregulated miR‐486‐5p expression, and miR‐486‐5p inhibited RCC cell proliferation and apoptosis resistance induced by CCL2. The study demonstrates that there are potential diagnosis and therapy values of miR‐486‐5p in RCC.

Từ khóa


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Thông tin xuất bản

Journal of Cellular Biochemistry

Tập 120 Số 3

2954-2963

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