Role of glucocorticoids in the chromaffin‐neuron developmental decision

International Journal of Developmental Neuroscience - Tập 7 - Trang 475-483 - 1989
David J Anderson1, Arie Michelsohn1
1Division of Biology 216-76, California Institute of Technology Pasadena, CA 91125, U.S.A.

Tóm tắt

AbstractChromaffin cells and sympathetic neurons develop from a common neural crest‐derived progenitor cell. The developmental fate of this cell differs depending upon whether it migrates to the sympathetic ganglion or to the adrenal gland primordium, suggesting that local environmental signals control its differentiation. Glucocorticoid (GC) is a good candidate for an important adrenal environmental signal. These steroids are known to regulate PNMT, an adrenal‐specific enzyme. However, in vivo observations suggest that the adrenal microenvironment influences the phenotype of sympathoadrenal progenitor cells as early as E14.5, 2 days before PNMT is first expressed by developing chromaffin cells. Using cDNA probes, we find that GC receptor mRNA can be detected in the embryonic adrenal at least one full day before the initial appearance of PNMT mRNA. This observation is compatible with the idea that the apparent early influence of the adrenal microenvironment reflects the action of GC on progenitors which have migrated into this environment. In support of this, we show that similar influences can be exerted by GC on PC12 cells, which contain GC receptor mRNA but do not express or induce PNMT mRNA. Taken together, these data suggest that other factors in addition to the presence of the GC receptor may be necessary for the developmental appearance of PNMT expression.

Tài liệu tham khảo

Anderson D.J., 1988, Neural Development and Regeneration, 187, 10.1007/978-3-642-73148-8_17 Anderson D.J., 1989, Society for Developmental Biology, 47th Meeting Proceedings: Neural development 10.1016/0092-8674(85)90122-9 10.1016/0092-8674(86)90823-8 10.1073/pnas.85.19.7119 Batter D.K., 1988, The complete nucleotide sequence and structure of the gene encoding bovine phenylethanolamine N‐methyltransferase, J. biol. Chem. 10.1016/0012-1606(81)90423-1 10.1016/0012-1606(82)90319-0 10.1016/0092-8674(83)90335-5 Carnahan J., 1988, Markers for the early sympathoadrenal lineage, Soc. Neurosci. Abstr., 14, 319a 10.1016/0012-1606(79)90085-X 10.1523/JNEUROSCI.05-08-02119.1985 10.1523/JNEUROSCI.05-08-02143.1985 10.1073/pnas.73.7.2424 10.1016/0165-3806(85)90030-6 10.1016/0166-2236(81)90056-4 10.1126/science.3952494 10.1128/MCB.7.9.3156 Lewis E.J., 1983, Regulation of tyrosine hydroxylase mRNA by glucocorticoid and cyclic AMP in a rat pheochromocytoma cell line, J. biol. Chem., 258, 14,632, 10.1016/S0021-9258(17)43909-3 10.1038/312779a0 10.1210/endo-81-4-716 Seidl K., 1988, Glucocorticoids initiate the differentiation of sympathoadrenal precursors to endocrine chromaffin cells, Eur. J. Neurosci. Abstr., 11, 123a 10.1016/0736-5748(89)90006-3 10.1016/0012-1606(88)90318-1 10.1016/0896-6273(88)90177-8 10.1016/0896-6273(88)90182-1 10.1016/0012-1606(82)90333-5 10.1073/pnas.75.7.3498 10.1007/BF00214643 Wurtman R.J., 1966, Control of enzymatic synthesis of adrenaline in the adrenal medulla by adrenal cortical steroids, J. biol. Chem., 241, 2301, 10.1016/S0021-9258(18)96620-2
Thông tin
Thông tin xuất bản

International Journal of Developmental Neuroscience

Tập 7

475-483

Thông tin tác giả