Role of foxj1 and estrogen receptor alpha in ciliated epithelial cell differentiation of the neonatal oviduct

Journal of Molecular Endocrinology - Tập 32 Số 3 - Trang 615-625 - 2004
Akinobu Okada1,2, Yoshihiro Ohta1, S.L. Brody3, Hajime Watanabe4, Andrée Krust5, Pierre Chambon5, Taisen Iguchi4
1Tottori University (4-101 Koyama-cho Minami, Tottori - Japan)
2Yamanouchi Pharmaceuticals (Yamanouchi Pharmaceuticals, Tsukuba - Japan)
3Washington University School of Medicine [Saint Louis, MO] (660 S. Euclid Ave., St. Louis, MO 63110-1010 - United States)
4CREST - Core Research for Evolutional Science and Technology (Japan Science and Technology Agency, 5 Sanbancho, Chiyoda-ku, Tokyo, 102-0075, Japan - Japan)
5IGBMC - Institut de génétique et biologie moléculaire et cellulaire (I.G.B.M.C, Parc D'Innovation 1 Rue Laurent Fries - BP 10142 67404 ILLKIRCH CEDEX - France)

Tóm tắt

Estrogen regulates proliferation and differentiation of epithelial cells in the mammalian oviduct, but pathways for cell-specific differentiation are not well understood. In the epithelial cells of the developing rat oviduct, we found estrogen receptor (ER) alpha is expressed at birth and persists in all cells through neonatal day (ND) 7 when ciliated cells appear. To determine a specific function of ER and foxj1, a transcription factor known to have fundamental roles in ciliogenesis in the lung, in differentiation of the ciliated epithelial cells, we treated newborn rats from ND 0 to 5 with estradiol-17beta (E2) with and without a selective ER antagonist. E2 enhanced the number of proliferating cells and accelerated the process of epithelial cell differentiation resulting in ciliogenesis by ND 5, and co-treatment with an ER antagonist inhibited these changes. Foxj1 was expressed only in the infundibulum and ampulla (INF/AMP). That expression preceded the appearance of cilia and was induced by E2. Cilia were absent in oviducts of foxj1-deficient mice, indicating that foxj1 plays a critical role in oviductal ciliogenesis. However, we found the presence of cilia in the ERalpha-deficient mouse oviduct. The widespread expression of ERalpha in oviductal epithelium, but restriction of cilia to the INF/AMP regions, and importantly, the presence of cilia in the ERalpha-deficient mice, suggested ER signaling is not essential for ciliated epithelial cell differentiation. These observations demonstrate that, although E2 stimulates the differentiation process of ciliated epithelial cells, foxj1 is directly required for epithelial cell ciliogenesis of the neonatal oviduct.

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Journal of Molecular Endocrinology

Tập 32 Số 3

615-625

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