Role of Mac‐1 integrin in generation of extracellular vesicles with antibacterial capacity from neutrophilic granulocytes

Journal of extracellular vesicles - Tập 9 Số 1 - 2020
Ákos M. Lőrincz1, Balázs Bartos1, Dávid Szombath1, Viktòria Szeifert1, Csaba I. Tímár1, Lilla Turiák2, László Drahos2, Ágnes Kittel3, Dániel Sándor Veres4, Ferenc Kolonics1, Attila Mócsai1, Erzsébet Ligeti1
1Department of Physiology, Semmelweis University, Budapest, Hungary
2MS Proteomics Research Group, Research Centre for Natural Science, Hungarian Academy of Sciences, Budapest, Hungary
3Experimental Research Institute of Hungarian Academy of Sciences, Budapest, Hungary
4Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary

Tóm tắt

ABSTRACTProduction of extracellular vesicles (EVs) involved in intercellular communication is a common capacity of most cell types. Upon encountering opsonized microorganisms, neutrophilic granulocytes release EVs that compromise bacterial growth. We carried out a systematic investigation of the involvement of potential opsonin receptors in EV‐generation from human and murine neutrophils. Applying flow cytometric, proteomic and functional analysis as well as using genetically modified mice, we demonstrate that formation of antibacterial EVs depends upon stimulation of the multifunctional Mac‐1 integrin complex, also called as complement receptor 3 (CR3), whereas activation of immunoglobulin binding Fc receptors or pattern recognition receptors alone or in combination is ineffective. Mac‐1/CR3 stimulation and downstream tyrosine kinase signalling affect both the numbers, the cargo content and the antibacterial capacity of the produced vesicles. In contrast, Mac‐1/CR3 signalling is not required for spontaneous EV formation, clearly indicating the existence of separate molecular pathways in EV biogenesis. We propose that EVs are “tailor‐made” with different composition and functional properties depending on the environmental circumstances.

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Journal of extracellular vesicles

Tập 9 Số 1

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