Vai trò của Interferon-γ trong hoạt động điều hòa miễn dịch của tế bào gốc màng xương người

Stem Cells - Tập 24 Số 2 - Trang 386-398 - 2006
Mauro Krampera1, Lorenzo Cosmi2, Roberta Angeli2, Anna Fratta Pasini1, Francesco Liotta2, Angelo Andreini1, Veronica Santarlasci2, Benedetta Mazzinghi2, Giovanni Pizzolo1, Fabrizio Vinante1, Paola Romagnani2, Enrico Maggi2, Sergio Romagnani2, Francesco Annunziato2
1Department of Clinical and Experimental Medicine, Section of Haematology, University of Verona, Verona
2Excellence Center of the University of Florence, DENOthe, Florence, Italy

Tóm tắt

Tóm tắt

Các tế bào gốc trung mô (MSCs) ức chế sự phát triển của các tế bào lympho T không liên quan HLA đối với sự kích thích đồng loại, nhưng các cơ chế chịu trách nhiệm cho hoạt động này chưa được hiểu đầy đủ. Chúng tôi cho thấy rằng MSCs ức chế sự tăng sinh của cả tế bào lympho T CD4+ và CD8+, cũng như của các tế bào giết tự nhiên (NK), trong khi chúng không ảnh hưởng đến sự phát triển của các tế bào lympho B. Hiệu ứng ức chế sự phát triển của MSCs không liên quan đến sự thay đổi trong biểu hiện của các dấu hiệu hoạt hóa tế bào, sự kích thích apoptosis của tế bào, hoặc sự mô phỏng/tăng cường hoạt động của tế bào điều hòa T. Hoạt động ức chế của MSCs không phụ thuộc vào tiếp xúc và yêu cầu sự có mặt của interferon (IFN)-γ do các tế bào T và NK hoạt hóa sản xuất. Do đó, ngay cả các tế bào B đã được kích hoạt cũng trở nên nhạy cảm với hoạt động ức chế của MSCs trong bối cảnh có IFN-γ thêm vào từ bên ngoài. Hiệu ứng ức chế của IFN-γ liên quan đến khả năng kích thích sự sản xuất hoạt tính indoleamine 2,3-dioxygenase bởi MSCs, mà ngược lại ức chế sự phát triển của các tế bào T hoặc NK đã được kích hoạt. Các phát hiện này gợi ý rằng hiệu ứng có lợi đối với bệnh ghép chống chủ do đồng truyền với MSCs có thể do việc kích hoạt các tính chất điều hòa miễn dịch của MSCs bởi IFN-γ do tế bào T sản sinh.

Từ khóa


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