Rice‐produced MSP142 of Plasmodium falciparum elicits antibodies that inhibit parasite growth in vitro

Parasite Immunology - Tập 38 Số 10 - Trang 635-641 - 2016
Qiande Chen1, Wanqi Liang2, Feng Qian3, Biao Qian2, Jianping Cao1, Dingguo Zhang2, Yaobo Xu1, Lin-hua Tang1
1National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Tropical Diseases, Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, China
2State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
3Department of Rheumatology and Immunology, Changzheng Hospital, Second Military Medical University, Shanghai, China

Tóm tắt

SummaryMany malaria antigens contain multiple disulphide bonds involved in the formation of inhibitory B‐cell epitopes. Producing properly folded malaria antigens in sufficient quantities for vaccination is often a challenge. The 42‐kDa fragment of Plasmodium falciparum merozoite surface protein 1 (MSP142) is such a kind of malaria antigen. In this study, we investigated the expression of MSP142 in a rice system (9522, a cultivar of Oryza sativa ssp. japonica), which was used as a bioreactor for protein production. The MSP142 gene was synthesized according to rice‐preferred codons and transformed into rice plants via an Agrobacterium‐mediated method. The recombinant antigen was efficiently expressed in rice seeds with a level up to 1.56% of total soluble protein and was recognized by both the conformational monoclonal antibody 5.2 (mAb5.2) and the pooled sera of P. falciparum malaria patients. Rabbits were immunized intramuscularly with the purified MSP142 formulated with Freund's adjuvant. High antibody titres against MSP142 were elicited. The rabbit immune sera reacted well with the native protein of P. falciparum parasite and strongly inhibited the in vitro growth of blood‐stage P. falciparum parasites, demonstrating that transgenic rice can become an efficient bioreactor for the production of malaria vaccine antigens.

Từ khóa


Tài liệu tham khảo

10.1016/0166-6851(91)90128-S

10.1017/S0031182009990515

Holder AA, 1999, Merozoite surface protein 1, immune evasion, and vaccines against asexual blood stage malaria, Parasitologia, 41, 409

10.1006/expr.1997.4147

10.1016/0166-6851(95)00102-7

Egan A, 1997, Characterization of human T‐ and B‐cell epitopes in the C terminus of Plasmodium falciparum merozoite surface protein 1: evidence for poor T‐cell recognition of polypeptides with numerous disulfide bonds, Infect Immun, 65, 3024, 10.1128/iai.65.8.3024-3031.1997

10.1128/IAI.69.3.1536-1546.2001

10.1128/IAI.73.1.287-297.2005

10.1371/journal.pone.0008787

10.4049/jimmunol.180.3.1451

10.1371/journal.pctr.0020012

10.1371/journal.pone.0004708

10.1128/IAI.71.12.6766-6774.2003

10.1128/IAI.01679-05

10.1073/pnas.012590199

10.1016/0169-4758(93)90031-A

10.1084/jem.180.1.389

10.1007/s00253-013-5477-6

10.1016/S0168-9452(01)00555-6

10.1016/j.ijpara.2007.06.005

10.1111/j.1467-7652.2009.00447.x

10.1111/j.1467-7652.2010.00526.x

10.1111/j.1467-7652.2009.00479.x

10.1186/1475-2875-10-106

10.1371/journal.pone.0037179

10.1007/s00253-011-3772-7

10.1371/journal.pone.0079920

10.1007/s00253-012-4071-7

10.1128/AEM.00714-13

10.1002/biot.201400350

10.1111/pbi.12255

10.1093/intimm/dxu063

10.1093/nar/30.10.e43

10.1007/s11248-007-9135-6

Liu QQ, 1998, A highly efficient transformation mediated by Agrobacterium in rice (Oryza sativa L.), Acta Phytophysiol Sin, 24, 259

10.1007/s10592-005-9073-x

10.1007/s00299-003-0683-9

10.1186/1743-422X-7-89

10.4049/jimmunol.172.10.6167

10.1093/nar/15.23.9627

10.1073/pnas.90.24.11811

10.1016/S1473-3099(10)70157-2

Thông tin
Thông tin xuất bản

Parasite Immunology

Tập 38 Số 10

635-641

Thông tin tác giả