Rho activation is required for transforming growth factor‐β‐induced epithelial‐mesenchymal transition in lens epithelial cells

Cell Biology International - Tập 31 - Trang 1225-1230 - 2007
Hee Jun Cho1, Jiyun Yoo1
1Department of Microbiology, Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, South Korea

Tóm tắt

AbstractLens epithelial cells undergo epithelial‐mesenchymal transition (EMT) after injury as in cataract extraction, leading to fibrosis of the lens capsule. We have recently shown that TGF‐β‐induced EMT in lens epithelial cells depends on PI3 kinase/Akt signal pathway. In this report, we suggest Smad3 is necessary for TGF‐β‐induced EMT by showing that the expression of dominant‐negative Smad3 blocks the expression of α‐smooth muscle actin (α‐SMA) and morphological changes. We also show that TGF‐β induces a biphasic change in Rho activity, and that Y27632, a selective inhibitor of Rho effector ROCK, inhibits TGF‐β‐induced EMT in vitro and in vivo. We finally show that Smad3 activation and Rho signal activation is independent each other. All of these findings suggest that Rho/ROCK activation together with Smad3 is necessary for TGF‐β‐induced EMT in lens epithelial cells.

Tài liệu tham khảo

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Thông tin xuất bản

Cell Biology International

Tập 31

1225-1230

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