Rezeptorkonformationen in neuem Licht: GPCR-basierte FRET-Sensoren

Vilardaga JP, Bünemann M, Krasel C et al. (2003) Measurement of the millisecond activation switch of G protein-coupled receptors in living cells. Nat Biotechnol 21:807–812

Stryer L (1978) Fluorescence energy transfer as a spectroscopic ruler. Annu Rev Biochem 47:819–846

Jares-Erijman EA, Jovin TM (2003) FRET imaging. Nat Biotechnol 21:1387–1395

Griffin BA, Adams SR, Tsien RY (1998) Specific covalent labeling of recombinant protein molecules inside live cells. Science 281:269–272

Hoffmann C, Gaietta G, Bünemann M et al. (2005) A FlAsH-based FRET approach to determine G protein-coupled receptor activation in living cells. Nat Methods 2:171–176

Hoffmann C, Gaietta G, Zürn A et al. (2010) Fluorescent labeling of tetracysteine-tagged proteins in intact cells. Nat Protoc 5:1666–1677

Lohse MJ, Nuber S, Hoffmann C (2012) Fluorescence/bioluminescence resonance energy transfer techniques to study g-protein-coupled receptor activation and signaling. Pharmacol Rev 64:299–336

Zürn A, Zabel U, Vilardaga JP et al. (2009) Fluorescence resonance energy transfer analysis of α2a-adrenergic receptor activation reveals distinct agonist-specific conformational changes. Mol Pharmacol 75:534–541