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Khôi phục khả năng kháng gen chuyển giao trong Arabidopsis thaliana là có thể đảo ngược
Tóm tắt
Năm mươi phần trăm cây Arabidopsis thaliana chuyển gen kháng hygromycin không truyền đạt được kiểu hình kháng cho thế hệ sau. Tuy nhiên, toàn bộ gen chuyển giao vẫn được di truyền theo quy luật Mendel trong tất cả các trường hợp như quan sát qua phân tích Southern. Sự không nhất quán giữa kiểu gen và kiểu hình là kết quả của mức độ phiên mã giảm ở các thể chuyển gen nhạy cảm. Việc bất hoạt gen xảy ra ở các cây có sự tích hợp nhiều bản sao của DNA ngoại lai. Không tìm thấy mối tương quan rõ ràng giữa sự bất hoạt gen và quá trình methyl hóa các residue cytidine trong chuỗi gen chuyển giao. Các mẫu mô từ nhiều cây chuyển gen nhạy cảm đã phục hồi mức độ kháng hygromycin thấp trên môi trường phân lập callus. Các thế hệ tiếp theo được tạo ra bằng tự thụ phấn đều nhạy cảm. Ngược lại, sự phục hồi tự phát khả năng kháng hygromycin đã được quan sát ở các cây con có nguồn gốc từ việc giao phối chéo với các cây loại hoang dã hoặc một thể chuyển gen nhạy cảm khác. Việc giảm số lượng bản sao không phải là yêu cầu tiên quyết cho sự tái hoạt động tự phát. Kháng tính thường bị mất lại trong thế hệ tiếp theo. Như vậy, việc bất hoạt và tái hoạt động của gen chuyển giao là có thể đảo ngược.
Từ khóa
#Arabidopsis thaliana #gen chuyển giao #kháng hygromycin #bất hoạt gen #tái hoạt động gen #phiên mã #methyl hóa #tính di truyềnTài liệu tham khảo
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