Responses ofHeterodera glycinesandMeloidogyne incognitaInfective Juveniles to Root Tissues, Root Exudates, and Root Extracts from Three Plant Species

Plant Disease - Tập 102 Số 9 - Trang 1733-1740 - 2018
Congli Wang1,2, Edward P. Masler1,2, Stephen T. Rogers1,2
1Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China; and,
2United States Department of Agriculture, Agricultural Research Service, Mycology and Nematology Genetic Diversity and Biology Laboratory, Beltsville, MD, 20705

Tóm tắt

The infective juvenile (J2) stage of endoparasitic plant nematodes uses plant chemical signals, released from roots, to localize and infect hosts. We examined the behaviors of soybean cyst nematode (Heterodera glycines) and root-knot nematode (Meloidogyne incognita) J2 in the presence of root signals from marigold (Tagetes patula), soybean (Glycine max), and pepper (Capsicum annuum). Signals were obtained from sources commonly used in phytoparasitic nematode chemotaxis studies: root tips, root exudates, and root extracts. Root tips from each plant species attracted M. incognita but H. glycines was attracted only to soybean. In contrast, root exudates prepared from marigold, pepper, or soybean seedlings were attractive to H. glycines but were repellent to M. incognita. Root extracts had the same effect as exudates. Fractionation of exudates by reversed-phase high-performance liquid chromatography (HPLC) (acetonitrile [CH3CN] and 0.1% trifluoroacetic acid) revealed highly polar and less polar components affecting behaviors. Fractions eluting at 12% CH3CN from all three plants attracted H. glycines and repelled M. incognita. None of the less polar HPLC fractions (>15% CH3CN) affected H. glycines but those from G. max and T. patula repelled M. incognita. Differences among exudates and effects of fractionation on behavior are discussed.

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Tài liệu tham khảo

10.1007/s10886-009-9657-x

10.1016/j.tplants.2013.11.006

10.1146/annurev.arplant.57.032905.105159

10.1371/journal.pone.0055731

10.1146/annurev.phyto.40.032602.130045

10.1163/138855410X516760

10.1007/978-3-642-23047-9_1

10.1163/156854102765216704

10.1007/BF01020375

10.1163/156854111X612306

10.1007/s10886-012-0105-y

10.1094/MPMI-05-12-0107-R

10.1007/s11101-008-9105-5

10.1017/S0031182000059394

10.1016/j.apsoil.2010.09.005

10.1038/srep41282

10.1201/b12614-15

10.1111/mpp.12057

10.1111/j.1364-3703.2005.00306.x

10.1163/15685411-00003038

10.1007/978-94-007-0434-3_2

10.1021/jf303107j

10.1007/BF00988457

10.1094/PHYTO-95-0158

10.1146/annurev.phyto.34.1.181

10.1007/978-94-011-5596-0_4

10.1079/9781780641515.0246

10.1007/s10886-012-0118-6

Rawsthorne D., 1986, J. Nematol., 18, 379

10.1098/rsif.2010.0417

Sardanelli S., 1997, Suppl. J. Nematol., 29, 625

10.1007/s10886-009-9703-8

10.1371/journal.pone.0015148

10.1163/156854109X447024

Winter M. D., 2002, Parasitology, 125, 561, 10.1017/S0031182002002482

10.1163/156854106776179953

10.1163/15685411-00002856

10.1371/journal.pone.0154675

10.1094/PHYTO.2000.90.11.1239

Thông tin
Thông tin xuất bản

Plant Disease

Tập 102 Số 9

1733-1740

Thông tin tác giả