Repeated lumbar punctures within 3 days may affect CSF biomarker levels

Springer Science and Business Media LLC - Tập 16 Số 1 - 2019
Martin Olsson1, Johan Ärlig1, Jan Hedner2, Kaj Blennow1, Henrik Zetterberg3
1Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, House V, 431 80, Mölndal, Sweden
2Center for Sleep and Vigilance Disorders, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden
3Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, University of Gothenburg, Mölndal, Sweden

Tóm tắt

AbstractLumbar puncture (LP) is a common way of collecting cerebrospinal fluid (CSF) both in the clinic and in research. In this extension of a study on the relationship between sleep deprivation and CSF biomarkers for Alzheimer’s disease, we investigated CSF biomarker dynamics in relation to rebound sleep after sleep deprivation. Two LPs were performed within 3 days in 13 healthy volunteers. We noticed an unexpected sharp rise in biomarker concentrations in the second sample and therefore repeated the experiment, but without sleep intervention, in four additional individuals. The findings were similar in these subjects, suggesting an inherent methodological problem with repeated LPs. The result corroborates findings in studies with repeated CSF collection via indwelling lumbar catheters, and needs to be addressed in, for instance, pharmacodynamic studies employing these techniques.

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Springer Science and Business Media LLC

Tập 16 Số 1

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