Renal oncocytoma: a comparative clinicopathologic study and fluorescent in-situ hybridization analysis of 73 cases with long-term follow-up

Diagnostic Pathology - 2010
Marie Dvorakova1, Rajiv Dhir2, Sheldon Bastacky2, Kathleen Cieply3, Marie Acquafondata2, Carol R Sherer3, Tracy L Mercuri3, Anil V. Parwani2
1Department of Pathology, UPMC Presbyterian, 3550 Terrace Street, Pittsburgh, PA, 15261, USA
2Department of Pathology, UPMC Shadyside, 5230 Centre Avenue, Pittsburgh, PA, 15232, USA
3Insitu Laboratory, UPMC Montefiore, 3459 Fifth Avenue, Pittsburgh, PA, 15213, USA

Tóm tắt

Abstract Clinical studies have confirmed that renal oncocytoma (RO) is a benign neoplasm with excellent prognosis. In diagnostically challenging cases of renal oncocytic epithelial neoplasms, fluorescent in-situ hybridization (FISH) is increasingly being used and its ability to distinguish RO from chromophobe renal cell carcinoma (ChRCC) has been documented. In this study, we evaluated the differential diagnostic contribution of FISH in cases of RO. Clinicopathologic data and glass slides from 73 patients with RO were reviewed; 20 cases of ChRCC were included for comparison. FISH analysis of formalin-fixed, paraffin-embedded sections was performed using centromeric probes for chromosomes 1, 2, 7 and 17. FISH analysis revealed ROs had frequent loss of signal for chromosome 1 (56%) and 17 (44%). Tumors with more than one loss were common (41%) and 10% cases showed loss of all chromosomes examined. A total of 18% cases did not show any abnormality. Our study shows that chromosomal abnormalities in both ROs and ChRCCs are common with frequent loss of chromosomes 1 and 17. No association was found between overall patient survival and the extent of chromosomal abnormalities. FISH results, even those showing significant chromosomal abnormalities, should not alter the primarily morphology-based diagnosis of RO.

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