Relationships of Microsporidian Genera, with Emphasis on the Polysporous Genera, Revealed by Sequences of the Largest Subunit of RNA Polymerase II (RPB1)

Journal of Eukaryotic Microbiology - Tập 48 Số 1 - Trang 111-117 - 2001
Sarah A. Cheney1, Nathalie J. Lafranchi-Tristem2, Dorothée Bourges2, Elizabeth U. Canning2
1Department of Biology, Imperial College of Science, Technology and Medicine, London, UK
2Department of Biology, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK

Tóm tắt

ABSTRACT. Molecular data have proved useful as an alternative to morphological data in showing the relationships of genera within the phylum Microsporidia, but until now have been available only for ribosomal genes. In previous studies protein‐coding genes of microsporidia have been used only to assess their position in the evolution of eukaryotes. For the first time we report on the use of a protein‐coding gene, the A‐G region of the largest subunit of RNA polymerase II (RPB1) from 14 mainly polysporous species, to generate an alternative phylogeny for microsporidia. Using the amino acid sequences, the genera and species fell into the same main groupings as had been obtained with 16S rDNA sequences, but the RPB1 data provided better resolution within these groups. The results supported the pairings of Trachipleistophora hominis with Vavraia culicis and Pleistophora hippoglossoideos with Pleistophora typicalis. They also confirmed that the genus Pleistophora is not monophyletic and that it will be necessary to transfer Pleistophora ovariae and Pleistophora mirandellae into one or more other genera, as has already been effected for Pleistophora anguillarum.

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Thông tin xuất bản

Journal of Eukaryotic Microbiology

Tập 48 Số 1

111-117

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