Regeneration and characterization of somatic hybrid plants of Citrus sinensis (sweet orange) and Citrus micrantha, a progenitor species of lime
Tóm tắt
Most commercial citrus fruit species and cultivars are inter- and intra-specific hybrids. Conventional hybridization in citrus is largely handicapped by apomixis and long juvenility. As an alternative, somatic hybridization via protoplast fusion has been employed to create novel citrus germplasm. Witches' broom disease of lime (WBDL) emerged in Oman during the 1970s, which has been spreading to the neighboring countries. The disease is extremely destructive. A possible solution to the WBDL problem is to develop resistant hybrids. Resistance is available among the citrus relatives of lime i.e., sweet orange. Somatic hybrids combining sweet orange with lime have been produced but the fruit characteristics are different from lime. Herein, we report the development of somatic hybrids between a progenitor of lime (Citrus micrantha) and sweet orange (Citrus sinensis) in efforts to recreate a lime-like fruit using sweet orange as a donor of disease resistance gene(s). Successful somatic hybridization was verified by ploidy analyses using flow cytometry and RAPD analyses. This is the first report of using a progenitor species in somatic hybridization experiments in efforts to resynthesize an improved lime.
Tài liệu tham khảo
Barrett, H., 1992. An Autotetraploid of the Key Lime, Citrus auran-tifolia. Fruit Var J 46: 166–170.
Bove, J.M., M. Garnier, A.M. Mjeni & A. Khayrallah, 1988. Witches' broom disease of small-fruited acid lime trees in Oman: First MLO disease of citrus. Proc Int Organ Citrus Virol 10: 307–309.
Deng, X.X., J.W. Grosser & F.G. Gmitter Jr., 1992. Intergeneric somatic hybrid plants from protoplast fusion of Fortunella cras-sifolia cv Meiwa with Citrus sinensis cv Valencia. Sci Hort 49: 55–62.
Garnier, M., L. Zreik & J.M. Bove, 1991. Witches' Broom, a lethal mycoplasma disease of lime trees in the Sultanate of Oman and the United Arab Emirates. Plant Dis 75: 546–551.
Grosser, J.W. & F.G. Gmitter Jr., 1990a. Protoplast fusion and citrus improvement. Plant Breed Rev 8: 339–374.
Grosser, J.W. & F.G. Gmitter Jr., 1990b. Somatic hybridization of citrus with wild relatives for germplasm enhancement and cultivar development. Hort Sci 25: 147–151.
Grosser, J.W., F.G. Gmitter Jr. & J.L. Chandler, 1988a. Intergeneric somatic hybrid plants from sexually incompatible woody species: Citrus sinensis and Severinia disticha. Theor Appl Genet 75: 397–401.
Grosser, J.W., F.G. Gmitter Jr. & J.L. Chandler, 1988b. Intergeneric somatic hybrid plants of Citrus sinensis cv Hamlin and Poncirus trifoliata cv Flying Dragon. Plant Cell Rep 7: 5–8.
Grosser, J.W., G.A. Moore & F.G. Gmitter Jr., 1989. Interspecific so-matic hybrid plants from the fusion of 'Key' lime (Citrus auran-tifolia) with Valencia sweet orange (Citrus sinensis) protoplasts. Sci Hort 39: 23–29.
Grosser, J.W., P. Ollitrault & O. Olivares-Fuster, 2000. So-matic hybridization in citrus: An effective tool to facili-tate variety improvement. In Vitro Cell Dev Biol Plant 36: 434–449.
Guo, W.W. & X.X. Deng, 2001. Wide somatic hybrids of Citrus with its related genera and their potential in genetic improvement. Euphytica 118: 175–183.
Hodgson, R.W. 1967. Horticultural varieties of citrus. In: W. Reuther, H.J. Webber and L.D. Batchelor (Eds.), The Citrus Industry, Vol. 1, University of California Press, Berkeley, pp. 431–587.
Khan, I.A., 2000. Present status of lime and investigations on the Witches' Broom Disease of lime in Oman. In: F.S. Davies and L.W. Timmer (Eds.), Proc Int Soc Citricult IX Congr, pp. 935–938.
Khan, I.A., 2001. Somatic hybridization for introgression of witches' broom disease resistance into genetic background of lime. In: Y.A. AI-Shayji (Ed.), Biotechnology Applications for the Arid Regions, pp. 197–204.
Khan, I.A. & M.L. Roose, 1988a. Frequency and charac-teristics of nucellar and zygotic seedlings in three culti-vars of trifoliate orange. J Am Soc Hort Sci 113: 105– 110.
Khan, I.A. & M.L. Roose, 1988b. Nucellar embryony: Detection and importance. Punjab Fruit J 41: 1–9.
Khan, I.A., R.F. Lee & J. Hartung, 2003. Confirming seed trans-mission of witches' broom disease of lime. In: 8th Int Congr Plant Pathology, 2–7 February 2003, New Zealand, pp. 281 (abstract).
Malik, M.N., R.W. Scora & R.K. Soost, 1974. Studies on the origin of the lemon. Hilgardia 42: 361–382.
Miranda, M. T., Motomura, F. Ikeda, T. Ohgawara, W. Saito, T. Endo, M. Omura & T. Moriguchi, 1997. Somatic hy-brids obtained by fusion between Poncirus trifoliata (2x) and Fortunella hindsii (4x) protoplasts. Plant Cell Rep 16: 401– 405.
Moghal, S.M., A.D. Zidgali & S.S. Moustafa, 1998. Natural host range and reaction of Citrus species to Witches' Broom Disease of lime in Oman. Proc IPM Conf SQU, pp.143–154.
Moore, G.A., 1985. Factors affecting in vitro embryogenesis from undeveloped ovules of mature Citrus fruit. J Am Soc Hort Sci 110: 66–70.
Murashige, T. & D.P.H. Tucker, 1969. Growth factor requirements of citrus tissue culture. Proc First Int Citrus Symp 3: 1155–1161.
Nicolosi, E., Z.N. Deng, A. Gentile, S. Malfa, G. Continella & E. Tribulato, 2000. Citrus phylogeny and genetic origin of important species as investigated by molecular markers. Theor Appl Genet 100: 1155–1166.
Ollitrault, P., D. Dambier, C. Jaqumond, V. Allent & F. Luro, 1996. In vitro rescue and selection of spontaneous triploids by flow cytometry for easy peeler citrus breeding. Proc Int Soc Citricult 2000, pp. 254–258.
Scora, R.W. & M.N. Malik, 1970. Chemical characterization of citrus as a tool in phylogeny. Taxon 19: 215–228.
Swingle, W.T., 1967. The botany of Citrus and its wild relatives. In: W. Reuther, H.J. Webber & L.D. Batchelor (Eds.), The Citrus Industry, Vol. I, The University of California Press, Berkley. pp. 190–430.
Viloria, Z., J.L. Chandler & J.W. Grosser, 2000. Development of improved acid Citrus fruit cultivars via interploid hybridization. Proc Int Soc Citricult IX Congr 2000, pp. 955–958.