Rita Rezzani1, Sandra Tengattini, Francesca Bonomini, Francesca Filippini, Oľga Pecháňová, Rossella Bianchi, Ramaroson Andriantsitohaina
1Department of Biomedical Sciences and Biotechnology, Division of Human Anatomy, University of Brescia, Brescia, Italy
Tóm tắt
Flavonoids, polyphenol derivatives of plant origin, possess a
broad range of pharmacological properties. A number of studies
have found both pro/anti-apoptotic effects for many of these
compounds. For these reasons we investigated whether
ProvinolsTM, flavonoids obtained from red wine, have antiapoptotic properties. The investigations have been carried out in
rats treated with Cyclosporine A (CsA). In particular, four groups
of rats have been treated for 21 days with either olive oil (control
group), with CsA, with ProvinolsTM, or with CsA and ProvinolsTM
simultaneously. Oxidative stress, systolic blood pressure, body
weight, biochemical parameters and different markers of
pro/anti-apoptotic pathway were measured. CsA produced an
increase of systolic blood pressure, a decrease in body weight,
serum creatinine levels, urinary total protein concentration and
creatinine clearance. Moreover, CsA induced renal alterations and
the translocation of Bax and cytochrome c from cytoplasm to
mitochondria and vice versa. These changes activated the
caspase cascade pathway, that leads to morphological and
biochemical features of apoptosis. ProvinolsTM restored
morphological and biochemical alterations and prevented
nephrotoxicity. In conclusion, this study may augment our
current understanding of the controversial pro-/anti-apoptotic
properties of flavonoids and their molecular mechanisms.
