Red blood cell permeability to thiol compounds following oxidative stress

European Journal of Haematology - Tập 57 Số 3 - Trang 241-246 - 1996
Dalia Mazor1, Erez Golan2, V. Philip2, Maya Katz3, A. Jafe3, Zvi Ben‐Zvi4, Naomi Meyerstein2
1Dr Kaufmann Haematology Laboratory, Physiology Department, Beer-Sheva, Israel.
2The Dr Kaufmann Haematology Laboratory, Physiology Department, Faculty of Health Sciences, Ben‐Gurion University of the Negev, Beer‐Sheva, Israel
3Obstetrics and Gynecology Department, Faculty of Health Sciences, Ben‐Gurion University of the Negev, Beer‐Sheva, Israel
4Clinical Pharmacology Department, Faculty of Health Sciences, Ben‐Gurion University of the Negev, Beer‐Sheva, Israel

Tóm tắt

Abstract:  The permeability of red blood cells (RBCs) to thiol containing compounds, reduced glutathione (GSH) and N‐acetyl cysteine (NAC), has been studied in control adult and neonatal cells and after oxidative stress. NAC penetrates the cell membrane easily while GSH hardly permeates. We measured their capacity to enhance intracellular non‐protein thiols (NPSH), after inducing damage to the membrane by formation of defects. Diamide, phenazine methosulfate (PMS) and t‐butyl hydroperoxide (BHP) were chosen as exogenous oxidants, each inducing damage by a different mechanism. Our data indicate that although neonatal cells are more sensitive to oxidative stress, only membrane damage induced by diamide, renders adult and neonatal cells permeable to GSH. NAC treatment enhances thiol levels in cells exposed to oxidizing agents, as well as in control cells.

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