Reconstitution of light‐harvesting chlorophyll‐protein complexes with Photosystem II complexes in soybean phosphatidylcholine liposomes

Isolated Photosystem II (PS II) complexes and light‐harvesting chlorophyll‐protein complexes have been reconstituted, both separately and together, into soybean phosphatidylcholine liposomes. PS II activity, as measured by dichlorophenolindophenol reduction, increased by 70% following reconstitution of PS II complexes. Proteoliposomes containing both PS II and light‐harvesting chlorophyll‐protein complexes also exhibited enhanced quantum efficiencies at sub‐saturating light intensities. This indicates that the light‐harvesting complexes are interacting with the PS II complexes in proteoliposomes, to increase effectively their antenna size.