Recognition of Avirulence Gene AvrLm1 from Hemibiotrophic Ascomycete Leptosphaeria maculans Triggers Salicylic Acid and Ethylene Signaling in Brassica napus

Molecular Plant-Microbe Interactions - Tập 25 Số 9 - Trang 1238-1250 - 2012
Vladimír Šašek1,2, Miroslava Nováková3,2, Barbora Jindřichová2, Károly Bóka4, Olga Valentová3, Lenka Burketová2
1Department of Plant Protection, Czech University of Life Sciences Prague, Kamýcká 129, 165 21 Prague 6, Czech Republic;
2Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Karlovce 1a, 160 00 Prague 6, Czech Republic
3Department of Biochemistry and Microbiology, Institute of Chemical Technology Prague, Technická 5, 166 28 Prague 6, Czech Republic
4Department of Plant Anatomy, Eötvös Loránd University, Pázmány Péter sétány 1/C, H-1117, Hungary

Tóm tắt

Interaction of a plant with a fungal pathogen is an encounter with hundreds of molecules. In contrast to this, a single molecule often decides between the disease and resistance. In the present article, we describe the defense responses triggered by AvrLm1, an avirulence gene from a hemibiotrophic ascomycete, Leptosphaeria maculans, responsible for an incompatible interaction with Brassica napus. Using multiple hormone quantification and expression analysis of defense-related genes, we investigated signaling events in Rlm1 plants infected with two sister isolates of L. maculans differentiated by the presence or absence of AvrLm1. Infection with the isolate carrying AvrLm1 increased the biosynthesis of salicylic acid (SA) and induced expression of the SA-associated genes ICS1, WRKY70, and PR-1, a feature characteristic of responses to biotrophic pathogens and resistance gene–mediated resistance. In addition to SA-signaling elements, we also observed the induction of ASC2a, HEL, and CHI genes associated with ethylene (ET) signaling. Pharmacological experiments confirmed the positive roles of SA and ET in mediating resistance to L. maculans. The unusual cooperation of SA and ET signaling might be a response to the hemibiotrophic nature of L. maculans. Our results also demonstrate the profound difference between the natural host B. napus and the model plant Arabidopsis in their response to L. maculans infection.

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Tài liệu tham khảo

10.1094/Phyto-85-1525

10.1007/s00344-007-0013-5

10.1094/PHYTO.2001.91.1.70

10.1094/PHYTO.2002.92.10.1122

10.1007/s11103-008-9435-0

10.1046/j.1365-313X.2003.01927.x

10.1146/annurev.phyto.44.070505.143440

10.1007/s11103-010-9701-9

10.1126/science.1164627

10.1007/s10658-005-2108-9

10.1016/S0021-9673(02)00024-9

10.1016/S0981-9428(02)01383-9

10.1038/nrg2812

10.1007/s10658-005-2233-5

10.1094/MPMI-20-4-0459

10.1111/j.1365-313X.2004.02171.x

10.1007/s00294-004-0488-6

10.1111/j.1365-2958.2006.05076.x

10.1111/j.1365-3059.1987.tb02213.x

10.1105/tpc.8.10.1773

10.1007/BF00049334

10.1016/j.pbi.2009.06.007

10.1046/j.1365-313x.2001.01096.x

10.1016/j.envexpbot.2011.02.018

10.1038/nature05286

10.1094/MPMI-20-4-0335

10.1046/j.1365-313x.1998.00245.x

10.1074/mcp.M800156-MCP200

10.1105/tpc.016980

10.1094/MPMI-20-10-1308

10.1093/bioinformatics/bth254

10.1146/annurev.phyto.40.120501.101443

10.1105/tpc.7.11.1951

10.1093/jxb/erm239

10.1094/MPMI.2000.13.4.430

10.1111/j.1364-3703.2004.00228.x

10.1016/j.pbi.2010.05.003

10.1111/j.1365-2958.2008.06547.x

10.1104/pp.003954

10.1046/j.1364-3703.2001.00055.x

10.1111/j.1469-8137.2009.02763.x

10.1038/nchembio.164

10.1094/PHYTO-97-7-0794

10.1007/BF00014495

10.1094/PHYTO.1999.89.12.1200

10.1111/j.1364-3703.2005.00282.x

10.1038/ncomms1189

10.1016/j.pbi.2011.03.006

10.1094/MPMI-23-9-1130

10.1111/j.1365-313X.2006.02688.x

Steiner, A. A. 1984. The universal nutrient solution. Pages 633-649 in: Proceedings of the Sixth International Congress on Soilless Culture, Wageningen, The Netherlands.

10.1105/tpc.007591

10.1104/pp.106.079467

10.1371/journal.pgen.1000772

10.1146/annurev.phyto.050908.135202

10.1038/35107108

10.1073/pnas.95.13.7819