Reciprocal pathway between medullary visceral zone and hypothalamic supraoptic nucleus or paraventricular nucleus involved in hyperosmotic regulation

Cell Biology International - Tập 33 - Trang 475-482 - 2009
Zhijun Yang1,2, Zhiren Rao2, Xiaodan Jiang1, Hua Yuan2, Li Duan2, Liangwei Chen2, Ying Wang2, Ruxiang Xu1, Yanjun Zeng3
1Neuromedical Institute, Zhujiang Hospital of Southern Medical University, 253# Gongye Road, Guangzhou 510282, China
2Institute of Neuroscience, The Fourth Military Medical University, Xi'an 710032, China
3Biomechanics and Medical Information Institute, Beijing University of Technology, Beijing 100022, PR China

Tóm tắt

AbstractIn this study we try to simultaneously investigate the response of neurons and astrocytes of rats following hyperosmotic stimulation and test the possibility that the reciprocal pathways between medullary visceral zone (MVZ) and hypothalamic paraventricular nucleus (PVN) or supraoptic nucleus (SON). Hyperosmotic pressure animal model was established by administering 3% sodium chloride as drinking water to rats. The distribution and expression of the HRP retrogradely labeled neurons, Fos, tyrosine hydroxylase (TH) or vasopressin (VP) positive neuron and glial fibrillary acidic protein (GFAP) positive astrocytes in the MVZ, SON and PVN were observed by quadruplicate‐labeling methods of WGA‐HRP retrograde tracing combined with anti‐Fos, TH (or VP) and GFAP immunohistochemical technique. Fos positive neurons within the MVZ, PVN and SON increased markedly. There were also a large number of GFAP positive structures in the brain and their distribution pattern was fundamentally similar or analogous to Fos positive neurons in the above‐mentioned areas. The augmented GFAP reactivities took on hypertrophic cell bodies, thicker and longer processes. Quadruplicate immunohistochemical staining showed that a neuron could be closely surrounded by many astrocytes and they formed neuron–astrocytic complex (N–ASC). Fos+/TH+/HRP+/GFAP+ and Fos+/VP+/HRP+/GFAP+ quadruplicate labeled N–ASC could be found in the MVZ, PVN and SON, respectively. The present results indicated that the neurons and astrocytes might be very active following hyperosmotic pressure and N–ASC as a functional unit might serve to modulate osmotic pressure. There were reciprocal osmoregulation pathways between the MVZ and SON or PVN in the brain.

Tài liệu tham khảo

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Cell Biology International

Tập 33

475-482

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