Reappraisal of indications and limitations of oxime therapy in organophosphate poisoning

Human and Experimental Toxicology - Tập 16 Số 8 - Trang 466-472 - 1997
Franz Worek1, M. Bäcker1, Horst Thiermann1, L. Szinicz1, Ulrike Mast2, R. Klimmek2, Peter Eyer2
1Institut für Pharmakologie und Toxikologie, Akademie des Sanitäts- und Gesundheitswesens der Bundeswehr, Ingolstädter Landstraße 100, D-85748 Garching
2Walther-Straub-Institut für Pharmakologie und Toxikologie der Ludwig-Maximilians-Universität München, Nußbaumstraße 26, D-80336 München, Germany

Tóm tắt

1 In vitro studies with human erythrocyte acetylcholin esterase (AChE) and the mouse diaphragm model were performed to unravel the various microscopic reaction parameters that contribute to the dynamic equilibrium of AChE inhibition, ageing and reactivation. These data may help to define more precisely the indications and limitations of oxime therapy in organophosphate (OP) poisoning. 2 Diethylphosphoryl-AChE resulting from intoxications with parathion, chlorpyrifos, chlorfenvinphos, diazi non and other OPs is characterized by slow sponta neous reactivation and low propensity for ageing. This kind of phosphorylated enzyme is particularly sus ceptible to reactivation by oximes. 3 None of the oximes tested (pralidoxime, obidoxime, HI 6 and HLö 7) can be regarded as a universally suitable reactivator. Obidoxime turned out to be the most potent and most efficacious oxime in reactivating AChE inhibited by various classes of OP insecticides and tabun. Obidoxime, however, was inferior to HI 6 against soman, sarin, cyclosarin and VX. Pralidoxime was generally less potent. 4 The kinetic data of reactivation established for diethylphosphoryl-AChE of human red cells indicate that the usually recommended dosage to attain a plasma concentration of 4 μg/ml does not permit exploitation of the full therapeutic potential of the oximes, in particular of pralidoxime. However, in suicidal mega-dose poisoning, oximes, even at optimal plasma concentrations, may be unable to cope with the fast re-inhibition of reactivated AChE in the first days following intoxication. 5 It is suggested that oximes be administered by continuous infusion following an initial bolus dose as long as reactivation can be expected and until permanent clinical improvement is achieved.

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Thông tin
Thông tin xuất bản

Human and Experimental Toxicology

Tập 16 Số 8

466-472

Thông tin tác giả