Phương pháp Khuếch đại Isothermal bằng cách sử dụng Huỳnh quang Thời gian thực và Màu sắc Thị giác để xác định Nhanh chóng và Rõ ràng Giống Diodon

Food Analytical Methods - Tập 15 - Trang 2487-2495 - 2022
Ruibin Xie1, Jie Gao1, Hui Li1, Wenjie Yu1, Juan Zhang1, Nan Wang1, Ailiang Chen1
1Institute of Quality Standard & Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, China

Tóm tắt

Trong nghiên cứu hiện tại, các phương pháp khuếch đại isothermal (LAMP) bằng huỳnh quang thời gian thực (flo-LAMP) và quang màu thị giác (vis-LAMP) đã được thiết lập để nhận diện Diodon. Các mồi LAMP được thiết kế cho gen cytochrome oxidase I của Diodon, và tính đặc hiệu cao của chúng đã được xác thực khi so sánh với 21 loài cá khác. LAMP có giới hạn phát hiện tối thiểu là 0.5 pg/μL đối với DNA genom. Hơn nữa, LAMP đã xác định được Diodon trong cả mẫu đã nấu chín và tiêu hóa, với kết quả cho thấy tiềm năng ứng dụng cao. Bộ phương pháp LAMP được tiến hành trong vòng 1 giờ dưới các điều kiện nhiệt độ không đổi. Đối với flo-LAMP, một kết quả dương tính được xác định thông qua đường cong khuếch đại hình chữ S. Đối với vis-LAMP, kết quả được quan sát bằng mắt thường trong ánh sáng ban ngày. Do đó, những phương pháp xác định Diodon nhanh chóng, đáng tin cậy, nhạy cảm và chi phí thấp này có thể được sử dụng để giám sát sự hiện diện của cá thuộc giống Diodon trong ngành thực phẩm và phát hiện các trường hợp ngộ độc Diodon.

Từ khóa

#LAMP #Diodon #khuếch đại isothermal #phát hiện nhanh #phương pháp màu sắc #ngộ độc cá

Tài liệu tham khảo

Aguilera O, Silva GOA, Lopes RT, Machado AS, Dos Santos TM, Marques G, Bertucci T, Aguiar T, Carrillo-Briceno J, Rodriguez F, Jaramillo C (2017) Neogene Proto-Caribbean porcupinefishes (Diodontidae). PLoS ONE 12(7):e0181670. https://doi.org/10.1371/journal.pone.0181670 Afrand M, Sourinejad I, Fazeli SA. S, Akbarzadeh A, Yeganeh L P, Sadeghi M & Azarbaijani R (2020, Feb 20). Morphological identification and molecular validation of anchovies (Engraulidae) in the Persian Gulf and Oman Sea. Zootaxa, 4742(2), zootaxa 4742 4742 4710. https://doi.org/10.11646/zootaxa.4742.2.10 Aravena P, Pulgar R, Ortiz-Severin J, Maza F, Gaete A, Martinez S, Seron E, Gonzalez M, & Cambiazo V, (2020, May 8). PCR-RFLP detection and genogroup identification of Piscirickettsia salmonis in field samples. Pathogens, 9(5). https://doi.org/10.3390/pathogens9050358 Aslan O, Hamill RM, Sweeney T, Reardon W, & Mullen AM (2009, Jan). Integrity of nuclear genomic deoxyribonucleic acid in cooked meat: implications for food traceability. J Anim Sci, 87(1), 57–61. https://doi.org/10.2527/jas.2008-0995 Cawthorn D-M, Steinman HA, Witthuhn RC (2011) Comparative study of different methods for the extraction of DNA from fish species commercially available in South Africa. Food Control 22(2):231–244. https://doi.org/10.1016/j.foodcont.2010.07.003 Chen SY, Zhang YX, Li H, Wang JH, Chen WL, Zhou Y, Zhou S (2014) Oct). Differentiation of fish species in Taiwan Strait by PCR-RFLP and lab-on-a-chip system. Food Control 44:26–34. https://doi.org/10.1016/j.foodcont.2014.03.019 Chow FW, Chan T T, Tam AR, Zhao S, Yao W, Fung J, Cheng F K, Lo G C, Chu S, Aw-Yong K L, Tang J Y, Tsang C C, Luk H K, Wong A C, Li K S, Zhu L, He Z, Tam E. W. T, Chung, T. W, Wong, S. C. Y., Que, T. L., Fung, K. S., Lung, D. C., Wu, A. K., Hung, I. F., Woo, P. C., & Lau, S. K (2020, Jul 29). A rapid, simple, inexpensive, and mobile colorimetric assay COVID-19-LAMP for mass on-site screening of COVID-19. Int J Mol Sci, 21(15), 5380. https://doi.org/10.3390/ijms21155380 Chuanjiang Z, Xi W, Qiaoge Z, Dongyan Z, Guoxian C, Yawei S, Dongying S, & Guoxing N (2019). Identification new fish record Henan province of Acheilognathus aff. Chankaensis based on morphology and mitochondrial COI gene sequence. Journal of Henan Normal University(Natural Science Edition), 47(5), 7–14. https://doi.org/10.16366/j.cnki.1000-2367.2019.05.002 Floren C, Wiedemann I, Brenig B, Schutz E, Beck J (2015) Apr 15). Species identification and quantification in meat and meat products using droplet digital PCR (ddPCR). Food Chem 173:1054–1058. https://doi.org/10.1016/j.foodchem.2014.10.138 Gatti C, Oelher E, Legrand AM (2008) Severe seafood poisoning in French Polynesia: a retrospective analysis of 129 medical files. Toxicon 51(5):746–753 Guan F, Jin YT, Zhao J, Xu AC, Luo YY (2018) A PCR method that can be further developed into PCR-RFLP assay for eight animal species identification. J Anal Methods Chem 2018:5890140. https://doi.org/10.1155/2018/5890140 Hulley EN, Tharmalingam S, Zarnke A, Boreham DR (2019) Development and validation of probe-based multiplex real-time PCR assays for the rapid and accurate detection of freshwater fish species. PLoS ONE 14(1):e0210165. https://doi.org/10.1371/journal.pone.0210165 Hwang D F, Hsieh Y W, Shiu Y C, Chen S K, & Cheng C A (2002, Feb). Identification of tetrodotoxin and fish species in a dried dressed fish fillet implicated in food poisoning. Journal of Food Protection, 65(2), 389–392. https://doi.org/10.4315/0362-028x-65.2.389 Kim MJ, Shin SW, Kim HB, Kim E, Kim HY (2021) Direct loop-mediated isothermal amplification (LAMP) assay for rapid on-site detection of Bifidobacterium longum subspecies longum, infantis, and suis in probiotic products. Food Chem 346(4):128887 Kubota R, Vine B G, Alvarez A M, & Jenkins D. (2008, Sep). Detection of Ralstonia solanacearum by loop-mediated isothermal amplification. Phytopathology, 98(9), 1045–1051. https://doi.org/10.1094/PHYTO-98-9-1045 Leis JM (2006) Nomenclature and distribution of the species of the porcupinefish family Diodontidae (Pisces, Teleostei). Memoirs of Museum Victoria 63(1):77 Li N, Shen Q, Wang J, Han C, Ji R, Li F, Jiang T (2015) Tetrodotoxin detection and species identification of pufferfish in retail roasted fish fillet by DNA barcoding in China. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 32(12):2148–2153. https://doi.org/10.1080/19440049.2015.1087056 Liang C, Liu Z, Liu C, Li Y, Yuan H, & Wang T (2018, Mar). Cook your samples: the application of microwave irradiation in speeding up biological processes. Mol Biotechnol, 60(3), 236–244. https://doi.org/10.1007/s12033-018-0061-z Liu K, Zhao S, Yu Z, Zhou Y, & Zhou C (2020). Application of DNA barcoding in fish identification of supermarkets in Henan province, China: more and longer COI gene sequences were obtained by designing new primers. Food Research International, 109516. Lucanoramírez G, Peñapérez E, Ruizramírez S, Vázquez J (2011) Reproduccion del pez erizo, Diodon holocanthus (Pisces: Diodontidae) en la plataforma continental del Pacifico Central Mexicano. Rev Biol Trop 59(1):217 Mahapatro D, Naik S, Behera DP, Mishra RK, Panda S (2015) First distributional record of an Indo-Pacific porcupine puffer fish Diodon holocanthus (Diodontidae) from the Gopalpur coast. Bay of Bengal Marine Biodiversity Records 8:e7 Minekus M, Alminger M, Alvito P, Ballance S, Bohn T, Bourlieu C, Carriere F, Boutrou R, Corredig M, Dupont D, Dufour C, Egger L, Golding M, Karakaya S, Kirkhus B, Le Feunteun S, Lesmes U, Macierzanka A, Mackie A, Marze S, McClements D J, Menard O, Recio, I., Santos, C. N., Singh, R. P., Vegarud, G. E., Wickham, M. S., Weitschies, W., & Brodkorb A (2014, Jun). A standardised static in vitro digestion method suitable for food - an international consensus. Food Funct, 5(6), 1113–1124. https://doi.org/10.1039/c3fo60702j Mori Y, & Notomi T (2020, Jan). Loop-mediated isothermal amplification (LAMP): expansion of its practical application as a tool to achieve universal health coverage. J Infect Chemother, 26(1), 13–17. https://doi.org/10.1016/j.jiac.2019.07.020 Nagamine K, Hase T, Notomi T (2002) Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes 16(3):223–229 Nagashima Y, Ohta A, Yin X, Ishizaki S, Matsumoto T, Doi H, & Ishibashi T (2018, Jan 8). Difference in uptake of tetrodotoxin and saxitoxins into liver tissue slices among pufferfish, boxfish and porcupinefish. Mar Drugs, 16(1). https://doi.org/10.3390/md16010017 Njiru Z K, Mikosza A S, Matovu E, Enyaru J C, Ouma J O, Kibona S N, Thompson R C, & Ndung'u J M (2008, Apr). African trypanosomiasis: sensitive and rapid detection of the sub-genus Trypanozoon by loop-mediated isothermal amplification (LAMP) of parasite DNA. Int J Parasitol, 38(5), 589–599. https://doi.org/10.1016/j.ijpara.2007.09.006 Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., & Hase, T. (2000, Jun 15). Loop-mediated isothermal amplification of DNA. Nucleic Acids Research, 28(12), 63. https://doi.org/10.1093/nar/28.12.e63 Panich W, Tejangkura T, & Chontananarth T (2021, Apr). Novel high-performance detection of Raillietina echinobothrida, Raillietina tetragona, and Raillietina cesticillus using loop-mediated isothermal amplification coupled with a lateral flow dipstick (LAMP-LFD). Vet Parasitol, 292, 109396. https://doi.org/10.1016/j.vetpar.2021.109396 Pappalardo A M, Petraccioli A, Capriglione T, & Ferrito V (2019, Jul 5). From fish eggs to fish name: caviar species discrimination by COIBar-RFLP, an efficient molecular approach to detect fraud in the caviar trade. Molecules, 24(13). https://doi.org/10.3390/molecules24132468 Prabhu V R, Singha H S, Kumar R G, Gopalakrishnan A, & Nagarajan M. (2020, May). Characterization of the complete mitochondrial genome of Barilius malabaricus and its phylogenetic implications. Genomics, 112(3), 2154–2163. https://doi.org/10.1016/j.ygeno.2019.12.009 Sukphattanaudomchoke C, Siripattanapipong S, Thita T, Leelayoova S, Ruang-Areerate T (2020) Simplified closed tube loop mediated isothermal amplification (LAMP) assay for visual diagnosis of Leishmania infection. Acta Trop 212:105651 Sultana, S., Ali, M. E., Hossain, M., Asing, Naquiah, N., & Zaidul, I. (2018). Universal mini COI barcode for the identification of fish species in processed products. Food Research International, 105(MAR.), 19–28. Sultana S, Hossain M A M, Azlan A, Johan M R, Chowdhury Z Z, & Ali M E (2020, Apr 7). TaqMan probe based multiplex quantitative PCR assay for determination of bovine, porcine and fish DNA in gelatin admixture, food products and dietary supplements. Food Chem, 325, 126756. https://doi.org/10.1016/j.foodchem.2020.126756 Trevett AJ, Mavo B, Warrell DA (1997) Tetrodotoxic poisoning from ingestion of a porcupine fish (Diodon hystrix) in Papua New Guinea: nerve conduction studies. Am J Trop Med Hyg 56(1):30–32. https://doi.org/10.4269/ajtmh.1997.56.30 Xiong X, Yuan F, Huang M, Cao M, & Xiong X (2020). Development of a rapid method for codfish identification in processed fish products based on SYBR Green real‐time PCR. International Journal of Food Science & Technology.