Rapid screening and identification of α‐glucosidase inhibitors from mulberry leaves using enzyme‐immobilized magnetic beads coupled with HPLC/MS and NMR

Biomedical Chromatography - Tập 27 Số 2 - Trang 148-155 - 2013
Tao Yi1, Yufeng Zhang1, Yiyu Cheng1, Yì Wáng1
1Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China

Tóm tắt

ABSTRACTα‐Glucosidase plays important roles in the digestion and absorption of carbohydrates in the small intestine. The inhibition of α‐glucosidase is regarded as a potential way to treat diabetes. We established an approach to screening α‐glucosidase inhibitors from medicinal plants using enzyme‐coated magnetic bead. Using 1‐(3‐dimethyl‐aminopropyl)‐3‐ethylcarbodiimide and N‐hydroxysuccinimide as reaction reagents, α‐glucosidase was immobilized on the magnetic beads by covalent linkage. The conjugation of α‐glucosidase to the magnetic beads was characterized using scanning electron microscope and X‐ray diffractometer. The proposed approach was applied in fishing potential α‐glucosidase inhibitors from extract of Morus alba, a Chinese medicinal plant. The structures of potential active compounds were identified via liquid chromatography–mass spectrometry and nuclear magnetic resonance. The results demonstrated that two flavonoids (isoquercitrin and astragalin) could bind to α‐glucosidase, which was confirmed via conventional α‐glucosidase inhibitory assay. Our findings suggested that enzyme‐coated magnetic beads may be suitable for discovering active compounds from medicinal plants. Copyright © 2012 John Wiley & Sons, Ltd.

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Thông tin xuất bản

Biomedical Chromatography

Tập 27 Số 2

148-155

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