Rapid concurrent analysis of fruit sugars and acids by gas‐liquid chromatography

Journal of the Science of Food and Agriculture - Tập 25 Số 9 - Trang 1095-1107 - 1974
D. A. Heatherbell1
1Processing and Pomology Section, Plant Disease Division D.S.I.R., Private Bag Auckland, New Zealand

Tóm tắt

AbstractA rapid and simple procedure for the separation, identification and quantitative analysis of common fruit sugars and non‐volatile organic acids has been developed. Acids were precipitated as their lead salts from fruit ethanolic extracts and the sugars in the remaining supernatant and washings were partitioned into aqueous methanol. These preparations with internal standards were dried and converted to their trimethylsilyl derivatives for analysis by gas‐liquid chromatography. In model studies, conditions which permitted full recovery of sugars from the lead salts of acids, while minimising the loss of acids, were investigated. For fruit acids reported present in measurable amounts (10 mg/100 g of fruit), replicate analysis of aliquots from the same extract was within ±5% for malonic, phosphoric, succinic, malic, citric, ascorbic and galacturonic acids and within ±12% for quinic acid. The use of the method, its advantages and limitations, in determining individual and total sugars and acids is illustrated on some New Zealand grown fruit (New Zealand grapefruit, mountain pawpaw, tangelo, apricot). Total sugars and total acids determined by this procedure gave comparable results with those obtained by using standard A.O.A.C. procedures.

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Tài liệu tham khảo

10.1016/0003-2697(64)90185-X

10.1016/0021-9673(64)80093-5

10.1111/j.1469-8137.1971.tb02527.x

Brunelle R. L., 1967, J. Ass. off. Analyt. Chem., 50, 329

Fitelson J., 1968, J. Ass. off. Analyt. Chem., 51, 1224

Fernandez‐Flores E., 1970, J. Ass. off. Analyt. Chem., 53, 17

Wagener W. W. D., 1971, Am. J. Enol. Vitic., 22, 167, 10.5344/ajev.1971.22.3.167

10.1111/j.1365-2621.1971.tb15550.x

10.1021/jf60177a006

10.1021/jf60185a018

Hodgson R. W., 1967, The Citrus Industry, 550

A.O.A.C. Methods of Analysis.Association of Official Agricultural Chemists Washington.196510th ed.

10.1021/jf60179a012

10.1021/jf60157a015

10.1093/chromsci/6.11.533

Stecher P. G., 1960, The Merck Index of Chemicals and Drugs, 604

10.1021/jf60180a033

Horning M. G., 1968, Analyt. Lett., 1, 713

10.1042/bj0290151

10.1080/00103627209366380

10.1021/jf60174a008

Ulrich R., 1970, Biochemistry of Fruits and their Products, 99

Ulrich R., 1970, Biochemistry of Fruits and their Products, 97

Romani R. J., 1971, Biochemistry of Fruits and their Products, 418

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Thông tin xuất bản

Journal of the Science of Food and Agriculture

Tập 25 Số 9

1095-1107

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