Gisèle Laguerre1, Marie-Reine Allard1, Françoise Revoy1, Noëlle Amarger1
1Laboratoire de Microbiologie des Sols, Institut National de la Recherche Agronomique, 21034 Dijon cedex, France
Tóm tắt
Forty-eight strains representing the eight recognized
Rhizobium
species, two new
Phaseolus
bean
Rhizobium
genomic species,
Bradyrhizobium
spp.,
Agrobacterium
spp., and unclassified rhizobia from various host plants were examined by restriction fragment length polymorphism (RFLP) analysis of 16S rRNA genes amplified by polymerase chain reaction (PCR). Twenty-one composite genotypes were obtained from the combined data of the RFLP analysis with nine endonucleases. Species assignments were in full agreement with the established taxonomic classification. Estimation from these data of genetic relationships between and within genera and species correlated well with previously published data based on DNA-rRNA hybridizations and sequence analysis of 16S rRNA genes. This PCR-RFLP method provides a rapid tool for the identification of root nodule isolates and the detection of new taxa.
