Rapid Extraction of Lycopene and β‐Carotene from Reconstituted Tomato Paste and Pink Grapefruit Homogenates

Journal of Food Science - Tập 55 Số 5 - Trang 1460-1461 - 1990
G. Sadler1, Jana Davis1, David J. Dezman1
1The authors are with the Univ. of Florida, Institute of Food & Agricultural Sciences, Citrus Research & Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850.

Tóm tắt

ABSTRACTSamples were extracted in Erlenmeyer flasks with hexane‐ethanol‐acetone (50:25:25). After extraction, 15 mL of water were added to separate the solution into polar and nonpolar layers. Lycopene was concentrated in the upper nonpolar phase while other components and polar pigments were contained in the lower polar phase. The lycopene phase was analyzed using isocratic HPLC with a C‐18 column and methanol‐THF‐water (67:27:6) mobile phase. Lycopene and (β‐caro‐tene eluted as chromatographically pure peaks.

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