Kỹ thuật bức xạ và phân hủy sinh học để giải độc dầu hạt Carica papaya nhằm sử dụng hiệu quả trong chế độ ăn uống và công nghiệp

Springer Science and Business Media LLC - Tập 52 - Trang 6475-6483 - 2015
Israel Sunmola Afolabi1, Tolulope Dorcas Bisi-Adeniyi1, Toluwalase Ronke Adedoyin1, Solomon Oladapo Rotimi1
1College of Science and Technology, Biological Sciences Department, Biochemistry Unit, Covenant University, Ota, Nigeria

Tóm tắt

Benzyl isothiocyanate (BITC) có độc tính ở nồng độ cao. Nghiên cứu khả năng của Aspergillus niger, bức xạ vi sóng và bức xạ cực tím trong việc giảm mức độ BITC trong dầu hạt Carica papaya Linn đã được tiến hành in vitro. BITC ở các nồng độ khác nhau đã được tiếp xúc định kỳ với bức xạ vi sóng và bức xạ cực tím trong 30 phút và 10 giờ, tương ứng; cùng với việc xác định Aspergillus niger trong 4 ngày. Bức xạ vi sóng đã làm giảm đáng kể (p < 0.05) mức BITC (0.0272, 0.0544 và 0.0816 μmol) xuống 12.19, 8.99 và 27.5% tương ứng trong vòng 15 phút. Bức xạ cực tím cũng làm giảm đáng kể (p < 0.05) mức BITC ở tất cả các nồng độ. A. niger đã làm tăng đáng kể (p < 0.05) sự phân hủy BITC vào ngày 2 và 4 với các giá trị lần lượt là 0.816, 1.36 và 2.72 nmol. Hoạt tính glutathione đã tăng đáng kể (p < 0.05) trong khi hoạt tính glutathione S-transferase đã giảm đáng kể (p < 0.05) ở tất cả các nồng độ vào ngày 3 và 4. Cả ba kỹ thuật đều có thể là các mô hình khả thi để cải thiện việc tiêu thụ dầu trong chế độ ăn uống.

Từ khóa

#Benzyl isothiocyanate #Carica papaya #Aspergillus niger #bức xạ vi sóng #bức xạ cực tím #phân hủy sinh học #giải độc dầu.

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